相衬显微镜实验设计  

Experimental design for phase contrast microscope

作　　者：朱昊[1] 郭宏[1] 曹良才[1] 任舟[1] 王佳[1] 

机构地区：[1]清华大学精仪系精密测试技术及仪器国家重点实验室,北京100084

出　　处：《实验技术与管理》2011年第10期31-33,37,共4页Experimental Technology and Management

摘　　要：曾获诺贝尔奖的相衬显微镜技术在光学发展史上具有里程碑意义,但现实中缺乏相关的教学实验。介绍了新开发的相衬显微镜实验的设计思想和实现方案,利用相衬附件在普通显微镜上进行相衬成像实验。学生在理论学习和掌握仪器操作的基础上,利用速干胶、洋葱表皮和酵母细胞自制简易标本,调节显微镜获得完美成像,以CCD及计算机采集并存储所获图像作为实验报告的素材,通过实验后的讨论加深理解,从而完成有个性的实验报告。The technology of phase contrast microscope which once won the Nobel physics prize has landmark significance in the history of the development of optics,but there are hardly any related teaching experiments in reality.The designing idea and implementation scheme of phase contrast microscope experiments newly developed are introduced.Phase contrast imaging is realized by using phase-contrast accessories on an ordinary microscope.Based on theoretical learning and handling instruments operation,the students use quick adhesive,onion epidermis and yeast cells to make simple specimen by themselves,adjust the microscope to obtain perfect images;use a CCD camera and a personal computer to collect and save images acquired as materials of experiment report.It is an attempt to develop interdisciplinary,research-oriented and opening experiment teaching.

关 键 词：相衬显微镜 实验设计 实验教学 

分 类 号：TH742.4[机械工程—光学工程] G642.423[机械工程—仪器科学与技术]