荸荠试管苗壮苗培育及小球茎再生研究  

Cultivation of vigorous plantlets and corm production from water chestnut test tube plantlets

作　　者：潘颖南[1] 杭玲[1] 陈丽娟[1] 张向军[1] 

机构地区：[1]广西农业科学院生物技术研究所,南宁530007

出　　处：《南方农业学报》2011年第10期1185-1188,共4页Journal of Southern Agriculture

基　　金：广西科技攻关项目(桂科攻10100008-5)

摘　　要：【目的】探讨荸荠试管苗壮苗方法和结球茎条件。【方法】以广西荸荠品种桂蹄1号试管苗为材料,分别进行不同蔗糖、PP333、温度等因素对荸荠试管苗壮苗及小球茎再生的影响。【结果】当6-BA偏低时(1.0mg/L),在不同培养基中添加30.0～120.0g/L蔗糖,均可不同程度诱导荸荠试管苗形成小球茎,其中以30.0g/L蔗糖的效果最好;当蔗糖浓度过高时则抑制小球茎形成。当6-BA偏高时(2.5mg/L),荸荠试管苗仅能诱导形成匍匐茎,而以添加80.0～100.0g/L蔗糖的效果较好。在添加NAA和IBA条件下,不同蔗糖量均能诱导荸荠试管苗长出匍匐茎,但添加30.0～60.0g/L蔗糖时可在匍匐茎顶端膨大形成小球茎,其中以30.0g/L蔗糖的诱导效果最好。在含有NAA和IBA的培养基中添加适宜的PP333(0.3～1.0mg/L)均能促进荸荠试管苗生根,并具有显著的壮苗效果,而对诱导荸荠试管结球茎数量影响不明显。在15～20℃条件下培养荸荠试管苗,添加蔗糖的培养基均未能诱导形成试管小球茎,而采用培养前20d温度28～30℃、培养后40d温度15～26℃条件,在培养基中添加生长素类或较低浓度6-BA时,含较低蔗糖量的培养基均能诱导较多的小球茎;当6-BA浓度较高时,仅能诱导荸荠试管苗长出匍匐茎。【结论】在MS+NAA0.5mg/L+IBA0.5mg/L+蔗糖30.0g/L+琼脂粉3.5g/L培养基中添加PP3330.3～1.0mg/L,均具有很好的壮苗效果。在适宜的温度条件下,添加适宜的6-BA或NAA和IBA或蔗糖(30.0g/L)均能诱导荸荠试管苗形成小球茎。[Objective]The aim of the experiment was to develop a method for obtaining vigorous test tube plantlets of water chestnut（Eleocharis tuberosa ） ,and to find out the best induction condition for their corms.[Method]The test tube plantlets of water chestnut variety Guiti 1 were used to study the effects of different concentrations of sucrose（30.0-120.0 g/L） ,PP333（0-1.0 mg/L） and temperatures on culturing vigorous test tube plantlets and reproducing corm.[Result]Addition of 1.0 mg/L 6-BA in culture medium containing 30.0-120.0 g/L sucrose resulted in induction of small corms in test tube plantlet.The best induction effects were observed at 30.0 g/L concentration of sucrose,over which the induction of corms was found to be inhibited.Addition of 2.5 mg/L 6-BA in culture medium resulted only in production of stolon.Similarly,addition of NAA,IBA and different concentrations of sucrose in culture medium resulted in development of stolon,but the corm induction from stolon occurred only in medium containing 30.0-60.0 g/L,and the treatment with 30.0 g/L sucrose was found most effective.Addition of NAA,IBA and different concentrations of PP333（0.3-1.0 mg/L） in culture medium resulted in induction of vigorous corm and roots,however quantity of induced corms did not differ in different treatments.Under culture temperature of 15-20℃ and adding auxin and lower 6-BA in medium,the plantlet could not induce the corm in all media adding sucrose.The corms could be induced in medium with lower sucrose under conditions of 28-30℃ for 20 days before culture and 15-26℃ for 40 days after culture.[Conclusion]The MS culture medium containing 0.5 mg/L NAA＋0.5 mg/L IBA ＋ 30.0 g/L sucrose ＋ 3.5 g/L agar supplemented with 0.3-1.0 mg/L PP333 showed the best effects on culturing vigorous plantlet.Adding suitable 6-BA or NAA and IBA or sucrose in culture medium could induce corm of plantlet.

关 键 词：荸荠 试管苗 壮苗 球茎 诱导 

分 类 号：S645.3[农业科学—蔬菜学]