L-365,260对吗啡成瘾大鼠尾核中痛兴奋神经元电活动的影响  

Effects of L-365,260 on the electrical activities of PEN in the caudate nucleus of morphinistic rats

作　　者：李晋峰[1] 成少华[2] 杨春晓[3] 

机构地区：[1]复旦大学附属上海市第五人民医院急诊科,上海200240 [2]复旦大学附属肿瘤医院闵行分院化疗科,上海200240 [3]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150081

出　　处：《中风与神经疾病杂志》2011年第10期883-886,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(30240058)

摘　　要：目的观察八肽胆囊收缩素(CCK-8)B受体拮抗剂L-365,260对正常及吗啡成瘾大鼠尾核(Cd)中痛兴奋神经元(PEN)电活动的影响,从而进一步探讨中枢CCK-8和尾核在吗啡成瘾大鼠痛觉调制中的作用。方法以电脉冲刺激大鼠坐骨神经作为伤害性痛刺激,用玻璃微电极记录尾核中PEN的放电,观察Cd内注入L-365,260对PEN电活动的影响。结果 L-365,260可降低吗啡成瘾与正常大鼠尾核中PEN的兴奋性,使PEN痛诱发放电频率减少,潜伏期延长。结论 L-365,260对吗啡成瘾及正常大鼠尾核中PEN均呈抑制作用。L-365,260是通过作用于尾核内CCK-B受体增强吗啡镇痛作用。间接证明CCK-8确实参与了大鼠中枢痛觉的调制,CCK-8主要通过激活大鼠尾核中CCK-B受体来下调吗啡镇痛作用的。Objective To study the effects of L-365,260 on the electrical activities of pain excitation neurons（PEN） in the caudate nucleus（Cd）of normal and morphinistic rats,accordingly further investigate the modulation of cholecystokinin-octopeptide（CCK-8） and caudate nucleus on central painful sense of morphinistic rats.Methods The sciatic nerve was stimulated by train of electrical impulses as noxious stimuli.The electrical discharges of PEN in the caudate nucleus were recorded with glass microelectrode to observe the influences of microinjection of L-365,260 into caudate nucleus on the electrical activities of PEN.Results L-365,260 could attenuate the excitabilities of PEN in the caudate nucleus of morphinistic and normal rats.Conclusion L-365,260 can attenuate the excitabilities of PEN in the caudate nucleus of morphinistic and normal rats to the electrical stimulation,displaying the inhibitive pain effect of L-365,260.CCK-8 probably by activation of rat caudate nucleus in the CCK-B receptors reduced the analgesic effect of morphine.

关 键 词：L-365 260 吗啡成瘾 尾核 痛兴奋神经元 

分 类 号：R741[医药卫生—神经病学与精神病学]