转化生长因子β-1对神经元树突生长以及突触素1表达的影响  

The effects of TGF-β1 on neuronal dendritic growth and the expression of synapsin1

作　　者：桂韦[1] 余传勇[1] 汪凯[1] 王玉[1] 

机构地区：[1]安徽医科大学第一附属医院神经内科,安徽合肥230022

出　　处：《中风与神经疾病杂志》2011年第10期887-890,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.30970997);安徽省自然科学基金资助项目(No.09020103008)

摘　　要：目的研究不同浓度转化生长因子β-1(TGF-β1)对体外培养的胎鼠小脑神经元树突生长及突触素1(synapsin1)表达的影响,探讨脑损伤后促进神经功能恢复的机制。方法取孕18d小鼠神经元细胞进行体外原代培养,在培养7d的小脑神经元中加入不同浓度的TGF-β1(1ng/ml、5ng/ml、10ng/ml),并设正常对照组。作用48h后,对培养的神经元细胞采用免疫细胞化学荧光标记:用结构性微管相关蛋白-2(microtubule associated protein-2,MAP2)标记树突,同时以突触素1抗体标记突触素1。用荧光显微镜直接观察细胞树突的长度变化;应用多功能酶标仪测量突触素1的荧光强度值。结果在1ng/ml、5ng/ml及10ng/ml TGF-β1干预培养48h后,小脑神经元树突长度分别为106.2±5.5、164.3±7.49、218.3±13.5,与对照组(63.7±7.8)相比,明显增长(P<0.05);TGF-β1也可促进突触素1的表达,在1ng/ml、5ng/ml、10ng/ml TGF-β1干预培养的条件下,突触素1荧光强度值分别为21940.7±870.0、38450.0±559.1、46067.5±1303.5,较对照组(17608.3±1389.3)显著增强(P<0.01)。结论TGF-β1可通过促进小脑神经元树突生长以及突触素1表达的增多,促进神经功能的恢复;其促进效应总体趋势呈剂量依赖性特征。Objective To investigate the effects of TGF-β1 on neuronal dendritic growth and the expression of synapsin1 and analyze the mechanism of neurological function recovery after brain injury.Methods The cells were derived from 18 days embryonic mouse.Dose-dependent effects of TGF-β1（1ng/ml to 10ng/ml） on cerebellar neurons were evaluated after 48 hours.The expression of microtubule associated protein-2（Map2） and synapsin1 was examined by immunocytochemistry staining.Map2 was used to observe the neurite growth of dendritic.Synapsin1 was used to measure the expression changes of synapsin1 by Multifunction Microplate Reader.Results At concentrations of 1ng/ml,5ng/ml and 10ng/ml TGF-β1,the length of cerebellar neuronal dendritic cultured for 48h was 106.2±5.5,164.3±7.49,218.3±13.5,respectively,which was significantly promoted compared with the control group（63.7±7.8）（P0.05）.Each dose group of TGF-β1 can increase the expression of synapsin1.Meanwhile,the value of synapsin1 fluorescence intensity was 21940.7±870.0,38450.0±559.1,46067.5±1303.5,respectively,which significantly increased compared with the control group（17608.3±1389.3）（P0.01）.Conclusion TGF-β1 can promote recovery of neurological function by promoting the growth of dendrites of cerebellar neurons and the increase of synapsin1 expression,which shows dose-dependent characteristics.

关 键 词：TGF-Β1 神经元 树突 生长 Synapsin1 

分 类 号：R741[医药卫生—神经病学与精神病学]