常见赤潮藻类的变性梯度凝胶电泳分析  

Analysis of harmful algal bloom species using denaturing gradient gel electrophoresis(DGGE)

作　　者：邵娟[1] 王朝晖[1] 林朗聪[1] 张珂[1] 

机构地区：[1]暨南大学水生生物研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2011年第5期504-508,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金资助项目(41076093);中央高校基本科研业务费专项资金项目(21611417)

摘　　要：为了解我国典型有毒有害赤潮藻类的变性梯度凝胶电泳(DGGE)指纹图谱以及DGGE技术在赤潮藻类分析鉴定中的作用,本课题组运用DGGE技术,研究了我国沿海7种重要赤潮藻类的单一种类以及混合种类样品的18S rDNA V3区的DGGE指纹图谱,并且对2009年10月底在广东省珠海海域发生的双胞旋沟藻(Cochlodini-um geminatum)赤潮样品进行了DGGE分析.结果表明,DGGE技术能够对环节环沟藻、海洋原甲藻、血红哈卡藻、锥状斯氏藻、中肋骨条藻、塔玛亚历山大藻、双胞旋沟藻7种常见的海洋赤潮藻类具有较好的区分效果,同时监测出赤潮样品中双胞旋沟藻、血红哈卡藻、环节环沟藻和海洋原甲藻4种优势种,种类组成与显微镜观察结果具有较好的一致性.结果说明DGGE技术可作为一种辅助方法对赤潮藻类进行分类鉴定.Denaturing gradient gel electrophoresis （DGGE） fingerprints of 18S rDNA V3 region of single species and multi-species of seven harmful algal bloom （HAB） taxa were studied in this study. The purpose is to understand DGGE fingerprints of typical HAB species from Chinese sea areas and the use of DGGE in the identification of HAB specie. A nature sample from the Cochlodinium geminatum bloom in Zhuhai coasts in October 2009 was analyzed. Results showed that distinguish lanes appeared in DGGE fingerprints by the seven HAB species including Gyrodinium instriatum, Prorocentrum micans, Akashiwo sanguinea, Scrippsiella trochoidea, Skeletonema costatum, Alexandrium tamarense and Cochlo- dinium geminatum. Meanwhile four dominant species （ Cochlodinium geminatum, Akashiwo sanguinea, Gyrodinium instriatum and Prorocentrum micans） were observed in fingerprint of the bloom sample, which were consistent with the microscopical observation. The results suggested that DGGE technique could be applied as an auxihary method for HAB species identification and analyzing.

关 键 词：变性梯度凝胶电泳(DGGE) 海洋微藻 双胞旋沟藻 赤潮 聚合酶链式反应(PCR) 

分 类 号：Q178.53[生物学—水生生物学] X55[生物学—普通生物学]