高效毛细管电泳法同时测定减肥类功能食品中7种违禁成分  

Simultaneous Determination of Seven Adulterants in Slimming Functional Foods by High Performance Capillary Electrophoresis

作　　者：李阳[1] 薛丰[1] 王媛[1] 薛勇[1] 孙成均[1] 

机构地区：[1]四川大学华西公共卫生学院,成都610041

出　　处：《分析化学》2011年第11期1716-1720,共5页Chinese Journal of Analytical Chemistry

基　　金：科技部"十一五"科技支撑计划资助项目(No.2006BAD27B02)

摘　　要：建立了同时测定减肥类功能食品中违禁成分芬氟拉明、伪麻黄碱、去甲伪麻黄碱、安非拉酮、两布曲明、两地那非和十的宁的高效毛细管电泳分析方法。以20mmol／L硼砂10mmol／LSDS-5％乙腈溶液（pH9．0）为运行缓冲液，在25℃，17kV条件下测定，检测波长为195nm。各待测物线性范围上限均为100mg／L，线性相关系数均大于0．998，相对标准偏差为4．5％～7．9％（n=7）；平均加标回收率为79．6％～112．0％；检出限（S／N3）为0．16～0．65mg／L。本方法简便快速、灵敏准确、分析成本低，已成功应用于功能食品中上述减肥类违禁添加成分的测定。pseudoep ming fun SDS-5% A method for the simultaneous determination of seven adulterants including fenfluramine, hedrine, norpseudoephedrine, amfepramone, sibutramine, sildenafil and strychnine in slimctional foods has been established. A mixture of 20 mmol/L sodium tetraborate-10 mmol/L acetonitrile was used as running buffer （pH 9.0）. The samples were analyzed by high performance capillary elctrophoresis under conditions 195 nm. The upper limits of linear ranges were all ter than 0. 998. The RSDs were 4. 5%- 7. 9% of 17 kV; 25 ℃ with the detection wavelength of 100 mg/L with linear correlation coefficients grea（n=7）. The average recoveries were 79. 6%-112.0%. The detection limits （S/N=3） were 0.16-0.65 mg/L. The method tive, accurate, low cost and has been successfully applied to the determination cals in the slimming functional foods. slm the e, fast, sensi target chemi

关 键 词：高效毛细管电泳法 减肥功能食品 违禁成分 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.8[轻工技术与工程—食品科学与工程]