阿米洛利对食管癌EC9706细胞侵袭能力的影响及其作用机制  

Effect of amiloride on the invasion capacity of esophageal carcinoma EC9706 cell line and its possiblemechanisms

作　　者：姜建涛[1] 周斌[1] 张淑群[2] 李少民[1] 张潍[1] 张晋[1] 乔哲[1] 孔冉冉[1] 马跃峰[1] 

机构地区：[1]西安交通大学医学院第二附属医院胸外科,710004 [2]西安交通大学医学院第二附属医院肿瘤科,710004

出　　处：《肿瘤研究与临床》2011年第10期657-660,共4页Cancer Research and Clinic

基　　金：陕西省科学技术研究发展计划（2009K12-01）

摘　　要：目的 研究阿米洛利对人类食管癌EC9706细胞体外侵袭能力的影响,探讨其可能的作用机制.方法 食管癌细胞EC9706经阿米洛利处理后,采用Transwell小室法检测其对细胞侵袭能力的影响,采用反转录聚合酶链反应（ RT-PCR）及Western blot法分别检测阿米洛利对细胞尿纤溶酶原激活物（ uPA） mRNA及蛋白相对于β-actin表达的影响.结果 经10、20、40、80 μmol/L阿米洛利作用24h后,EC9706细胞体外侵袭人工重组基膜的能力降低,并随着阿米洛利浓度的增加,EC9706细胞的穿膜细胞数由对照组的（96±7）个分别下降为（78±6）、（57±6）、（33±4）、（15±3）个（F=43.46,P＜ 0.01）.随着阿米洛利浓度的增加,uPA mRNA的表达由对照组的（0.623±0.065）分别下降为（0.526±0.054）、（0.389±0.041）、（0.312±0.038）、（0.247±0.025）（F=6.71,P＜ 0.01）,uPA蛋白由对照组的（ 0.732±0.064）分别下降为（0.644±0.057）、（0.533±0.058）、（0.391±0.036）、（0.267±0.043）（F=6.71,P＜ 0.01）.结论 阿米洛利能抑制食管癌细胞的侵袭能力。Objective To investigate the effect of amiloride on the invasion capacity of esophageal carcinoma EC9706 cell line in vitro and to elucidate its possible mechanism.Methods The invasion capacities of EC9706 cells pretreated with amiloride were measured by transwell chamber assay. The urokinase-type plasminogen activator （uPA） transcription were determined by RT-PCR.The protein expression of uPA were assessed by Western blot.Results After the EC9706 cells were pretreated with amiloride at different concentrations,the number of invaded cells was obviously less than those of control group with obvious dosage dependent pattern （96±7,78±6,57±6,33±4,15±3,F =43.46,P 〈 0.01）.The transcription levels of uPA mRNA and the protein expression levels of uPA in EC9706 cells decreased significantly compared with the control （mRNA：0.623±0.065,0.526±0.054,0.389±0.041,0.312±0.038,0.247±0.025,F =6.71,P 〈0.01; protein：0.732±0.064,0.644±0.057,0.533±0.058,0.391±0.036,0.267±0.043,F =6.71,P 〈0.01）.Conclusion Amiloride inhibits the invasion capacity of esophageal carcinoma EC9706 cells.The mechanism might be associated with down-regulation of the expression of uPA

关 键 词：食管肿瘤 侵袭 尿纤溶酶原激活物 肿瘤细胞 培养的 阿米洛利 

分 类 号：R735.1[医药卫生—肿瘤]