生长激素促进结直肠癌细胞增殖的实验研究  

Study on promotion of growth hormone for colorectal cancer cell proliferation

作　　者：吴晓宇[1] 葛亮[2] 陈志伟[1] 姚学权[1] 陈彻[1] 许哲[1] 李为苏[1] 刘福坤[1] 

机构地区：[1]南京中医药大学附属江苏省中医院消化肿瘤外科,210029 [2]南京中医药大学附属江苏省中医院检验科,210029

出　　处：《肿瘤研究与临床》2011年第10期671-673,677,共4页Cancer Research and Clinic

摘　　要：目的 研究生长激素受体（GHR）在结直肠癌细胞株表面的表达情况并探讨人类重组生长激素（rh-GH）对人类结直肠癌细胞增殖的影响.方法 采用流式细胞术及免疫荧光法检测9株人类结直肠癌细胞株表面GHR表达；四甲基偶氮唑蓝比色（MTT）法检测不同浓度rh-GH以及生长激素受体拮抗剂（GHRA）对结直肠癌细胞增殖的影响.结果 GHR在多株结直肠癌细胞株表面存在表达差异,其中GHR在HCT-8人类结直肠癌细胞高表达[（58.23±5.51 ）％],LoVo细胞几乎不表达[（0.20±0.04） ％].一定质量浓度的r-hGH（ 50、100 ng/ml）对GHR＋的HCT-8细胞有促增殖作用（均P＜0.05）,但并不呈剂量依赖；应用GHRA封闭GHR后,r-hGH的促增殖作用消失；而rhGH对GHR-的人类结肠癌细胞株LoVo增殖无影响（均P＞0.05）.结论 外源性生长激素对人类结直肠癌细胞有促增殖作用。Objective To detect the expression of GHR in colorectal cancer cell lines and determine whether recombinant human growth hormone can promote the proliferation of colorectal cancer cells in vitro.Methods GHR distribution was assessed by flow cytometry and immunofluorescence method in 9 colorectal cancer cell lines.The effect of recombinant human growth factor on colorectal cancer cell line proliferation was assessed by MTT method.Results Different GHR expression was determinated in 9 colorectal caner cell lines.GHR was highly expressed in HCT-8 while GHR expression could hardly be detected in LoVo.r-hGH could promote GHR（＋） HCT-8 cell proliferation at 50 ng/ml and 100 ng/ml （P 〈0.05）.But this effect was not dose dependent.When the neutralizing antibody was used to block GHR activity,this r-hGH dependent proliferation effect was eliminated.r-hGH could not promote GHR （-） LoVo cell proliferation （P 〉0.05）.Conclusion The results demonstrates that r-hGH could promote GHR （＋） tumor cell proliferation and this effect is mediated by GHR.The use of r-hGH on the colorectal cancer patients should be cautious

关 键 词：结直肠肿瘤 生长激素 生长激素受体 

分 类 号：R735.3[医药卫生—肿瘤]