亚低温治疗对大鼠脑梗死后Bax和Bcl-2表达的影响  

Influence of mild hypothermia on the expression of Bax and Bcl-2 after acute cerebral infarction in rats

作　　者：王玉凯[1] 任丽[2] 陶恩祥[3] 

机构地区：[1]中山大学附属佛山医院神经内科,广东佛山528000 [2]济宁医学院附属医院 [3]中山大学第二附属医院

出　　处：《卒中与神经疾病》2011年第5期271-274,共4页Stroke and Nervous Diseases

基　　金：佛山市科技局医学类科技攻关项目(NO:0408024)

摘　　要：目的探讨亚低温对大鼠急性脑梗死Bax、Bcl-2表达的影响。方法清洁级(SPF)雄性健康SD大鼠,采用线栓法建立局灶性脑梗死模型,分别于缺血3、6、12 h给予亚低温治疗,缺血24 h取材观察。实验分常温组(大鼠10只)、亚低温3、6、12 h组(每组大鼠各10只),假手术组(大鼠6只),用TTC染色测定脑梗死体积,用免疫组化的方法检测Bax、Bcl-2的表达水平。结果与常温组比较,亚低温组脑梗死体积明显减少(P<0.01)、Bcl-2表达上调、Bax表达下调(P<0.05)。亚低温组脑梗死体积3 h<6 h<12 h组,Bcl-2表达3 h>6 h>12 h、Bax表达3 h<6 h<12 h(P<0.05)。结论亚低温治疗可能通过抑制Bax和Bcl-2表达,从而抑制神经元凋亡、降低脑梗死体积,脑缺血后越早期给予亚低温治疗效果越好。Objective To observe the influence of mild hypothermia on the expression of Bax and Bcl-2 after acute cerebral infarction in rats. Methods Focal cerebral infarction models were induced in male healthy SD Rats（SPF scale） using intraluminal thread insertion.Rats were treated with mild hypothermia 3,6 and 12 hours after cerebral ischemia and experiment objects were taken at 24 hours after cerebral ischemia.The rats were randomly divided into normothermic group（10 Rats）,mild hypothermic 3h group（10 Rats）,6h group（10 Rats）,12h group（10 Rats） and sham operation group（6 Rats）.The infarct volume was detected with 2,3,5-triphenyl-tetrazolium chloride staining.Immuno-histochemical analysis was used to detect the expressions of Bax and Bcl-2 of each group. Results Compared with normothermic group,mild hypothermia significantly reduced infarct volume increased while Bax significantly the expression of Bcl-2 and decreased the expression of Bax.Among mild hypothermic groups,infarct volume is 3 h6 h12 h（P0.05）,the expressions of Bax is 3 h6 h12 h and Bax-2 is 3 h6 h12 h（P0.05）. Conclusions Uprequlation of Bax and downregulation of Bcl-2 may be an important mecharasm underlying the neuroprotective effect of mild hypotheria against ischemic brain injury.The earlier mild hypothermia therapy,the better the result.

关 键 词：亚低温 脑梗死 大鼠 BAX BCL-2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]