促红细胞生成素对大鼠实验性视网膜脱离细胞凋亡的干预作用  

Interferential effect of erythropoietin on cell apoptosis in rat experimental retinal detachment

作　　者：刘骁[1] 唐罗生[2] 段国平[1] 

机构地区：[1]湖南省人民医院眼科,湖南省长沙市410005 [2]中南大学湘雅二医院眼科,湖南省长沙市410011

出　　处：《眼科新进展》2011年第11期1029-1032,共4页Recent Advances in Ophthalmology

摘　　要：目的探讨促红细胞生成素(erythropoietin,EPO)对实验性视网膜脱离大鼠细胞凋亡的干预作用。方法选用SD大鼠44只,随机分为正常对照组(4只)、生理盐水(normal saliue,NS)对照组20只和EPO治疗组(20只)。正常对照组不做任何处理,其他两组大鼠建立孔源性视网膜脱离模型。造模前4hEPO治疗组大鼠腹腔中注射EPO,NS对照组注射生理盐水。术后1h、6h、12h、24h、72h处死大鼠,每个时间点各取4只,摘取眼球,TUNEL染色,计算内核层、节细胞层及外核层细胞数,进行统计学分析。结果正常对照组大鼠视网膜层次结构清晰,各层排列整齐。NS对照组在脱离后12h连续层状结构紊乱,外节丢失增多,而EPO治疗组的连续层状结构存在,外节变性较轻。TUNEL染色结果显示EPO治疗组在视网膜脱离后1h、12h、24h、72h节细胞层及内核层凋亡细胞数[分别为(4.75±1.42)/目、(7.17±1.27)/目、(9.41±1.08)/目、(11.83±1.11)/目]少于NS对照组(7.83±1.70)/目、(9.67±1.56)/目、(12.42±1.31)/目、(15.25±1.22)/目,差异有统计学意义(均为P<0.001)。而外核层EPO治疗组在视网膜脱离后12h、24h、72h的凋亡细胞数[分别为(33.08±8.64)/mm2、(49.17±12.52)/mm2、(92.83±14.14)/mm2]少于NS对照组,差异有统计学意义(均为P<0.05),余时段2组间比较差异均无统计学意义。结论通过腹腔注射EPO能减少实验性视网膜脱离所致视网膜各层细胞的凋亡。Objective To study the interferential effect of erythropoietin（EPO） on cell apoptosis in rat experimental retinal detachment（RD）.Methods Forty-four SD rats were chosen and divided into 3 groups：normal control group（4 cases）,EPO treated group（20 cases） and normal saline（NS） control group（20 cases）.No any treatment was performed in normal control group,the animal RD model was established in EPO treated group and NS control group.EPO or NS was injected into peritoneal cavity at 4 hours before experimental RD models establishment.The rats were sacrificed at 1 hour,6 hours,12 hours,24 hours and 72 hours after RD,4 cases in each time point.The eyeballs were extracted and stained by TUNEL,the cell numbers in the inner nuclear layer,ganglion cell layer and outer nuclear layer were calculated and statistical analyzed.Results The retinal layers in normal control group were clear and arranged regularly.The continuous retinal layers were confused,and the lost outer ganglion cells increased at 12 hours after RD in NS control group,but in EPO treated group,the continuous retinal layers were clear,the degeneration outer ganglion cells were slight.The apoptotic cell numbers in retinal ganglion cell layer and inner nuclear layer at 1 hour,12 hours,24 hours and 72 hours after RD in EPO treated group were（4.75±1.42）/2.5 mm2,（7.17±1.27）/2.5 mm2,（9.41±1.08）/2.5 mm2 and（11.83±1.11）/2.5 mm2,respectively,which were less than that in NS control group,there were statistical differences（all P〈0.001）.The apoptotic cell numbers in retinal outer nuclear layer at 12 hours,24 hours and 72 hours after RD in EPO treated group were（33.08±8.64）/mm2,（49.17±12.52）/mm2 and（92.83±14.14）/mm2,respectively,which were less than that in NS control group,there were statistical differences（all P〈0.05）.Conclusion EPO injecting into peritoneal cavity can reduce the retinal cell apoptosis induced by experimental RD.

关 键 词：视网膜脱离 促红细胞生成素 凋亡 TUNEL 

分 类 号：R774[医药卫生—眼科]