Avastin玻璃体腔注射对小鼠氧诱导视网膜病变新生血管的抑制作用  

The inhibitory effects of avastin on retinal angiogenesis in mice models after intravitreal injection

作　　者：付浴东[1] 周占宇[1] 万金娥[1] 丰慧[1] 李松涛[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003

出　　处：《中华实验眼科杂志》2011年第11期988-992,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景早产儿视网膜病变（ROP）主要源于视网膜新生血管的形成，avastin可以同血管内皮生长因子（VEGF）的所有异构体发生高亲和力结合，拮抗其生理活性。目的观察玻璃体腔注射avastin对氧诱导视网膜病变模型新生血管的作用。方法取7日龄清洁级C57BL／6J小鼠90只，用随机数字表法随机分为6组：空气对照组、高氧对照组、高氧BSS组和低、中、高剂量avastin组，每组15只。空气对照组小鼠在空气中喂养，其他各组均置于体积分数75％±2％O，的高氧环境中饲养，连续5d，高氧BSS组大鼠玻璃体腔注射无菌BSS液0．5μl，3个avastin处理组小鼠分别玻璃体腔注射1．25、2．50、5．00g／Lavastin各0．5μl。17日龄时过量麻醉处死小鼠，摘除眼球制备视网膜组织切片，苏木精一伊红染色后计数突破内界膜的新生血管内皮细胞核数目；应用免疫组织化学染色法检测视网膜中CD34分子的表达；利用视网膜铺片技术观察低、中、高剂量avastin组大鼠视网膜新生血管的形态并进行比较。结果苏木精一伊红染色发现，高氧对照组小鼠视网膜突破内界膜的新生血管内皮细胞核数目明显多于空气对照组，差异有统计学意义（P〈0．01）；不同剂量的avastin组小鼠视网膜中突破内界膜的新生血管内皮细胞核数明显少于高氧BSS组和高氧对照组，差异均有统计学意义（P〈0．01）；高剂量avastin组与低剂量avastin组比较，突破内界膜的新生血管内皮细胞核数明显减少，差异有统计学意义（P〈0．05）。免疫组织化学检测显示，高氧对照组视网膜突破内界膜的内皮细胞CD34分子呈阳性表达。视网膜铺片可见空气对照组的血管自视盘向四周均匀放射，分支良好，结构清晰；高氧对照组、高氧BBS组可见大量新生血管，其结构紊乱，分布不均；各剂量avastin组随着剂量的增加，视网膜血管分布和走行�Background Retinopathy of prematurity is mainly due to retinal neovaseularization. Objective This laboratory work was to evaluate the efficacy of different dosage of avastin for inhibiting retinal neovascularization. Methods Ninety 7-day-old clean C57BL/J6 mice were randomized into six groups as follows： air control group, hyperxia control group,hyperxia BSS group and avastin groups. C57BL/J6 mice in air control group were raised in regular air environments. The fifty mice were fed under the environment with 75% ±2% oxygen for 5 days to establish the retinal neovascularization models. The 1.25,2.50 and 5. 00 g/L avastin （0.5μl） were injected inteavtreally in forty-five mice models as low,moderate and high dosage avastin groups respectively, and 0. 5 μl BSS was used at the same way in fifteen models as hyperxia BSS group. The mice were sacrificed in the 17-day-old age using excessive anesthesia method and the retina sections were prepared for the calculation of the numbers of vascular endothelial cell nuclei broken retinal inner membrane after hemotoxylin and eosin staining. The expression of CD34 in the retina was detected by immunochemistry. The morphology and distribution of retinal neovascular vessel in various groups were observed using retinal flat. The use of the animals followed the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission. Results The numbers of cell nuclei broken the inner limiting membrane was significant increased in the hyperxia grotlp compared with the air control group（ P〈0.01 ）, and those in difference doses of avastin were considerably reduced in comparison with hyperxia BSS group （P〈0.01） and hyperxia group （P〈0.01）. The decrease of numbers of cell nuclei broken the inner limiting membrane was obvious in low dose of high dose of avastin compared with low dose of avastiu （ P〈0. 05）. CD34 was positively expressed in retina internal membrane of hyperxia group. Retinal flat revealed the re

关 键 词：AVASTIN 视网膜/氧诱导视网膜新生血管化 血管形成 玻璃体腔注射 

分 类 号：R774.1[医药卫生—眼科] R988.1[医药卫生—临床医学]