蒿甲醚对C6胶质瘤细胞的抑制作用  

Inhibitory effect of artemether on C6 glioma cells

作　　者：邓兴力[1] 颜小荣[1] 李宣鹏[1] 王波[1] 魏小兵[1] 李杨[1] 李玉[1] 

机构地区：[1]昆明医学院第一附属医院神经外科,650032

出　　处：《中国微侵袭神经外科杂志》2011年第11期514-517,共4页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的研究蒿甲醚对大鼠C6胶质瘤细胞的抑制作用。方法 C6胶质瘤细胞经培养后,按是否加入蒿甲醚分为实验组和对照组,均采用四甲基偶氮唑盐(MTT)法、流式细胞技术(FCM)及Hoechst33258荧光染色法检测细胞凋亡情况。结果MTT法检测显示:24、48、72 h 3个时间组蒿甲醚对C6胶质瘤细胞的半数抑制浓度(IC50)分别为(195.08±3.27)μmol/L、(119.64±4.06)μmol/L、(87.84±0.93)μmol/L。FCM法检测显示:24、48、72 h 3个时间组C6胶质瘤细胞凋亡率分别为:7.95%、22.01%、31.22%;且G0-G1期细胞比例增加,S期和G2-M期细胞比例降低。荧光染色显示:实验组细胞内出现凋亡小体;而对照组细胞呈弥散均匀荧光。结论蒿甲醚能抑制C6胶质瘤细胞生长,且其抑制作用呈现时间依赖性和浓度依赖性;蒿甲醚能干扰C6胶质瘤细胞的细胞周期,可将其阻滞在G0-G1期并诱导其凋亡。Objective To research inhibitory effect of artemether on C6 glioma cells in rats. Methods According to whether adding the artemether, C6 glioma cells were divided into the experimental group and control group after cultivation. Cell apoptosis was detected by MTT method, flow cytometry （FCM） and Hoechst33258 fluorescence staining. Results 50% inhibit concentrations （IC50） of artemether to C6 glioma cells in the time group of 24, 48, 72h were 195.08±3.27 tzmol/L, 119.64±4.06 μmol/L, 87.84±0.93μmol/L respectively by MTT method. Cell apoptosis rates of C6 glioma cells in the time group of 24, 48, 72h were 7.95%, 22.01%, 31.22% respectively, and the cells percentage at G0-Gt phase increased and the cells percentage at S phase and G2-M phase decreased by FCM. Apoptotic body was seen in the cells of the experimental group, while diffuse and uniform fluorescence was observed in the control group by fluorescence staining. Conclusions Artemether can inhibit the growth of C6 glioma cells, and the inhibitory effect shows time dependence and concentration dependence. Al-temether can interfere with the cell cycle, and cause the C6 glioma c.ell arrest in G0-Gl phase and cell apoptosis.

关 键 词：神经胶质瘤 蒿甲醚 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]