溶血磷脂酰胆碱对牛视网膜微血管内皮细胞PDGF-B表达的影响  

Effects of lysophosphatidylcholine on the expression of platelet derived growth factor B in bovine retinal microvascular endothelial cells

作　　者：张朝云[1] 王辉[1] 林安华[2] 雷闽湘[2] 黄国栋[2] 宫学华[3] 

机构地区：[1]中国石油中心医院内分泌科,河北廊坊065000 [2]中南大学湘雅医院内分泌科,湖南长沙410008 [3]中国石油中心医院门诊部,河北廊坊065000

出　　处：《武警医学院学报》2011年第10期786-788,共3页Acta Academiae Medicinae CPAPF

摘　　要：【目的】探讨溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)对牛视网膜微血管内皮细胞(bovine retinal microvascularendothelial cells,BRECs)表达血小板衍生生长因子-B(platelet derived growth factor B,PDGF-B)的影响。【方法】原代培养的牛视网膜微血管内皮细胞分为4组:对照组,LPC(20、40、60μmol/L)3组;LPC各组根据不同作用时间(6、12、24 h)又分为3个亚组,用PDGF-B试剂盒检测各组内皮细胞培养上清液中PDGF-B蛋白的含量;用RT-PCR法检测PDGF-B mRNA的表达。【结果】LPC可使BRECs表达PDGF-B增加,具有时间和剂量依赖性,LPC(60μmol/L)作用12 h时效果最明显,差异有统计学意义(P<0.05)。【结论】LPC可引起BRECs表达PDGF-B增加,在糖尿病视网膜病变(diabetic retinopathy,DR)早期保护周细胞。【Objective】To study the effects of lysophosphatidylcholine（LPC） on the expression of platelet derived growth factor B（PDGF-B）in cultured bovine retinal microvascular endothelial cells（BRECs）.【Methods】The primary BRECs were divided into 4 groups： the control group and 3 LPC groups according to the doses used（20,40,60 μmol/L）.Each LPC group was divided again into 3 subgroups depending on incubation time.The PDGF-B in conditioned media in which BRECs were cultured was assessed with PDGF-B kit.RT-PCR was used to detect the PDGF-B mRNA expression.【Results】In the LPC groups,the PDGF-B increased significantly.The changes showed time and dosage dependence.The expression was highest after LPC（60 μmol/L）incubating BRECs for 12 hours（P 0.05）.【Conclusion】LPC could cause up-regulation of the expression of PDGF-B of endothelia cells,which may protect the pericytes loss in the early stage of diabetic retinopathy.

关 键 词：溶血磷脂酰胆碱 内皮细胞 血小板衍生生长因子 

分 类 号：R774.1[医药卫生—眼科]