与辣椒抗根结线虫基因Me1紧密连锁的EST-SSR标记开发  

DEVELOPMENT OF EST-SSR MARKERS LINKED TO ME1,A NEMATODES RESISTANCE GENE IN PEPPER

作　　者：张宇[1,2] 张晓芬[1] 陈斌[1] 耿三省[1] 李焕秀[2] 

机构地区：[1]北京市农林科学院蔬菜研究中心,北京100097 [2]四川农业大学园艺学院,四川雅安625014

出　　处：《核农学报》2011年第5期933-938,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家"863"项目(2006AA100108);国家科技支撑计划(2009BADB8B01);北京市科技计划(D08070500690803);北京市常规育种财政专项

摘　　要：以含抗根结线虫病基因Me1的PM217与感线虫品种茄门,及其F1、F2作为试验材料,采用南方根结线虫(Meloidogyne spp.)人工接种鉴定,根据鉴定结果,利用分离群体分组分析法(bulked segregantanalysis,BSA)建立抗感池,共筛选到3对(118、141及211)多态性EST-SSR引物在抗感池间存在差异。利用Joinmap3.0软件,结合抗病性鉴定,对F2群体进行SSR分析,研究3个EST-SSR标记位点与根结线虫抗性基因Me1的连锁关系,结果表明,EST-SSR标记141、118及211与Me1的遗传距离分别为6.984、18.684和29.310cM。本研究得到的与辣椒抗根结线虫显性单基因Me1紧密连锁的EST-SSR标记,为Me1基因的标记辅助选择提供了参考。In this study,we investigated the resistance to Meloidogyne spp with artifical inoculation systems among F2 lines derivered from susceptible variety Qiemen and resistant variety PM217 carried nematodes resistance gene Me1,with artificial Meloidogyne spp.inoculation systems.Based on the phenotype,we constructed a resistance bulk and a susceptible bulks to screen out the linkage markers.Using EST-SSR technology,the combination primers 118,141 and 211 were shown to be linked to Me1.Furthermore,with exponding population,the three markers were mapped to the locus with genetic distances of 6.984,18.684 and 29.310cM,respectively using Joinmap3.0 software.Identification of EST-SSR molecular markers linked to the dominant resistant gene Me1 laid laying the foundation for resistance breeding.

关 键 词：辣椒 根结线虫 Me1基因 EST-SSR 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]