水蛭素对大鼠DIC模型脑组织水通道蛋白4表达的干预作用  

Intervention of Hirudin on Aquaporin 4 Expression in Rat Brain with DIC

作　　者：玉光哲[1] 金英玉[2] 刘倩[2] 刘琰[2] 张艳芬[2] 辛晓敏[2] 张亚男[2] 彭鹏[2] 

机构地区：[1]哈尔滨医科大学附属第一医院急诊外科,哈尔滨150001 [2]哈尔滨医科大学附属第一医院检验科,哈尔滨150001

出　　处：《现代检验医学杂志》2011年第5期55-57,共3页Journal of Modern Laboratory Medicine

基　　金：教育部课题（项目编号：Z2009-1-15005）;黑龙江省中医药课题（项目编号：ZHY10-Z71）.

摘　　要：目的 探讨水蛭素对DIC模型的大鼠脑组织水通道蛋白4（AQP4）表达的干预作用.方法 通过鼠尾静脉滴注脂多糖制作DIC模型,将大鼠分为三组即对照组、DIC组和水蛭素组,选取4个时间点（4,6,8,12 h）测定血小板计数,PT,APTT,纤维蛋白原和D-D水平的变化;同时用免疫印迹杂交法检测大鼠脑组织水通道蛋白4的表达.结果 与对照组相比,DIC组及水蛭素组血小板计数,PT,APTT,纤维蛋白原和D-D差异具有统计学意义（P＜0.05）;水蛭素组上述指标变化明显低于DIC组（P＜0.05）;AQP4表达在水蛭素组明显低于DIC组（P＜0.05）.结论 DIC时产生的凝血酶可能通过上调AQP4蛋白的表达,参与脑水肿形成和发展;水蛭素通过抑制凝血酶的形成,减轻脑水肿.Objectives To assess intervention of hirudin on aquaporin 4（AQP4） expression in rat brain with DIC. Methods The rats were randomly divided into control group,DIC group,hirudin group,and DIC model was made by driping LPS in the rat tail. Platelet counts,PT,APTT,Fibrinogen and D-D were measured at 4,6,8 and 12 h time point. AQP4 was measured by western blot. Results Platelet counts,PT,APTT,Fibrinogen and D-D in DIC group and hirudin group were significantly higher or rower compared with the control group （P^0.05）. Those index of hirudin group were not less significant than DIC group （P〈0. 05）;AQP4 expression in hirudin group were lower than DIC group （P〈0. 05）. Conclusion Thrombin induced by DIC upregulate AQP4 expression which participate in cerebral edematization;hirudin inhibites the effect of thromin and encephaledema were relieved.

关 键 词：水蛭素 弥散性血管内凝血 水通道蛋白4 

分 类 号：R-332[医药卫生]