邻联甲苯胺法测定刺芹侧耳漆酶活力的影响因素  

Influential Factors of Laccase Activity from Pleurotus eryngii Determined by Ortho-tolidine Spectrometry

作　　者：李艳丽[1] 金周雨[1] 李玉[2] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]食药用菌教育部工程研究中心,长春130118

出　　处：《吉林农业大学学报》2011年第6期620-623,共4页Journal of Jilin Agricultural University

基　　金：吉林农业大学科研启动基金项目(201030)

摘　　要：系统地研究了邻联甲苯胺法测定刺芹侧耳漆酶活力的影响因素。结果表明:波长620 nm、反应温度30℃、pH 4.2、邻联甲苯胺浓度5 mmol/L、反应时间3 min时,刺芹侧耳漆酶活力测定的灵敏度最高,酶活力最大,为3 033 U/(min.mL),是筛选前测定酶活力的1.98倍。Influential factors of Laccase activity from trometry were investigated systematically. The results length was at 620 nm, temperature was at 30 °C, 5 mmol/L and reaction time was for 3 minutes when activity were the highest and laccase activity was up Pleurotus eryngii determined by ortho-tolidine specshowed that the optimum conditions were that wavepH was at 4.2, ortho-tolidine concentration was at both sensitivity rate of determination and the laccase to 3 033 U/（min mL）. It was 1.98 times of before.

关 键 词：漆酶 酶活力 邻联甲苯胺法 剌芹侧耳 

分 类 号：R284[医药卫生—中药学] Q949.329[医药卫生—中医学]