afamin在未破裂颅内动脉瘤患者血清中的表达及意义  

Expression of afamin in serum of patients with unruptured intracranial aneurysm and its significance

作　　者：朱永华[1] 杨有业[1] 林少华[1] 漆松涛[2] 黄汉添[1] 廖巍[1] 胡子慧[1] 李亮明[1] 林其昌[1] 罗勇[1] 

机构地区：[1]中山大学附属中山医院（中山市人民医院）神经外科,中山528403 [2]南方医科大学南方医院神经外科,广州510515

出　　处：《中华神经医学杂志》2011年第11期1115-1118,共4页Chinese Journal of Neuromedicine

摘　　要：目的寻找未破裂颅内动脉瘤患者较正常成人血清中表达差异的蛋白。方法选择中山大学附属中山医院（中山市人民医院）神经外科自2007年7月至2009年9月收治的未破裂颅内动脉瘤患者8例，同期体检健康者8例作为正常对照组，应用双向差异凝胶电泳（2-D DIGE）、基质辅助激光解析离子化飞行时间质谱（MALDI-TOF-MS）分析并鉴定2组血清中差异蛋白质的表达。结果与对照组比较，未破裂颅内动脉瘤患者血清中有29个表达差异在1．5倍以上的蛋白点，其中579蛋白点在未破裂颅内动脉瘤患者血清中比对照组表达降低1．81倍，差异有统计学意义（P=0．008），经MALDI-TOF-MS鉴定为afamin。结论afamin在未破裂颅内动脉瘤患者血清中的表达较低．可能参与了颅内动脉瘤的形成及扩张。Objective To search the differentially expressed proteins in the serum of patients with unruptured intracranial aneurysm. Methods Eight patients with unruptured intracranial aneurysm, admitted to our hospital from July 2007 to September 2009, and 8 healthy adults （controls） were recruited in our study; two-dimensional differential in-gel electrophoresis （2D DIGE） and matrix-assisted laser desorption/ionization - time of flight mass spectrometry （MALDI-TOF-MS） were employed to analyze and identify the differentially expressed proteins in the serum of these patients and controls. Results As compared with the control group, patients with unruptured intracranial aneurysm had 29 differentially expressed proteins those enjoyed more than 1.5 folds differences; protein at spot 579 was expressed as low as 1.81 folds in the serum of patients with unruptured intracranial aneurysm as compared with that in controls （P=0.008）; the protein was identified as afamin using MALDI-TOF-MS. Conclusion The low expression of afamin in patients with unruptured intracranial aneurysm probably contributes to the formation and expansion of intracranial aneurysm.

关 键 词：颅内动脉瘤 afamin 血清蛋白 双向差异凝胶电泳 

分 类 号：R739.41[医药卫生—肿瘤]