MG132和DDP联合应用对人鼻咽癌细胞CNE-1凋亡影响及其机制的探讨  

Apoptosis and machanism of CNE-1 treated with MG132 associated with DDP

作　　者：张学文[1] 朱逸[1] 钮洪艳[2] 

机构地区：[1]徐州医学院附属淮安市第二人民医院耳鼻喉科,江苏淮安223002 [2]徐州医学院附属淮安市第二人民医院中心实验室,江苏淮安223002

出　　处：《中华肿瘤防治杂志》2011年第18期1455-1457,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究蛋白酶体抑制剂MG132与顺铂(DDP)联合应用对人鼻咽癌细胞株CNE-1细胞凋亡的影响,观察细胞中凋亡相关蛋白Bcl-2和Bad表达的变化。方法:体外培养的CNE-1细胞分别暴露于MG132和DDP、单独MG132或者DDP,24h后,流式细胞术(FCW)检测CNE-1细胞的凋亡率,蛋白质印迹法测定CNE-1细胞中Bcl-2和Bad蛋白的表达情况。结果:流式细胞术检测结果显示,MG132和DDP联合用药组CNE-1细胞凋亡率(53±3.2)%较单独应用MG132组(20±1.7)%、DDP组(18±2.3)%的凋亡率显著增加。蛋白质检测结果显示,与MG132组、DDP组比MG132和DDP组Bcl-2的表达减少约3倍,而Bad的表达增加约2倍。结论:MG132和DDP联合应用可能通过降低Bcl-2同时增强Bad的表达而进一步诱导CNE-1细胞的凋亡。因此,我们可以认为MG132能够增强DDP对CNE-1细胞凋亡的作用。OBJECTIVE： To study the apoptosis of CNE-1 cell treated with proteasomes inhibitor MG132 associated with DDP, and observe the expression of Bcl-2 and Bad in CNE -1 cells. METHODS： CNE- 1 cells were exposed to MG132 and DDP, MG132 or DDP in vitro, 24 later, flow cy tometry detected the apoptosis of CNE-1 cells, and the expressoin of Bcl 2 and Bad was measured with Western Blot. RESULTS： The apoptosis of CNE-1 cells treated with MG132 and DDP（53± 3. 2）% simultaneously was increased significantly than that alone exposed to MG132（20± 1. 7）% and DDP（18± 2. 3）%. Meanwhile, the expression of Bcl-2 was decreased to reach 3 times and the expression of Bad was in creased to about 2 times in MG132 and DDP group compared with MG132 or DDP groups. CONCLUSIONS： MG132 associated with DDP can further induce CNE-1 cells apoptosis through depressing the expression of Bcl-2 and promoting Bad expression, thus, It may concludes that MG132 could enhance the effect of DDP to CNE-1 cells apoptosis.

关 键 词：鼻咽肿瘤 蛋白酶体抑制剂 顺铂 CNE-1细胞 

分 类 号：R739.63[医药卫生—肿瘤]