颅内肿瘤患儿血清细胞因子高通量液态芯片检测临床应用价值的探讨  

Detection of 5 serum cytokines in children with brain tumors by high throughput liquid chip technique

作　　者：荆楠[1] 康熙雄[1] 刘志忠[1] 周亚莉[1] 刘力[1] 刘淑静[1] 许宏[2] 

机构地区：[1]首都医科大学附属北京天坛医院检验科,北京100050 [2]首都医科大学附属北京儿童医院检验科,北京100045

出　　处：《中华肿瘤防治杂志》2011年第18期1462-1465,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家863重点课题子课题(2006AA020706-03)

摘　　要：目的:探讨IL-6、IL-8、IL-10、TNF-α和IFN-γ在颅内肿瘤患儿血清中的表达和各细胞因子之间相关性,以及它们与WHO肿瘤分级的关系。方法:采用高通量液态芯片法联合检测73例颅内肿瘤患儿(5～15岁)和42例健康儿童(5～15岁)血清中上述细胞因子的水平。结果:颅内肿瘤患儿血清IL-6、IL-8、IL-10、TNF-α和IFN-γ水平均显著高于健康对照组,P<0.01;各细胞因子之间具有相关性;IL-8在低级别肿瘤患儿血清中的水平显著高于其在高级别肿瘤患儿血清中的水平,P<0.05。结论:IL-6、IL-8、IL-10、TNF-α和IFN-γ可能参与儿童颅内肿瘤发病过程,各细胞因子之间存在相互作用,IL-8可作为预测颅内肿瘤级别的一个参考指标。OBJECTIVE： To explore the levels of serum interleu- kin （IL）-6, ILS, IL-10, tumor necrosis factor-a （TNF-a）, interferon-γ （IFN -γ） in children with brain tumors and correlations among them, and their association with the WHO tumor grade. METHODS： Seventy- three children（S-15 years old） with brain tumor and forty-two healthy controls（5-15 years old） were included. Serum levels of IL-6, IL-8, IL-10,TNF-a and IFN-γ of the two groups were measured by high throughput liquid chip technique. RESULTS： Serum levels of IL-6, IL-8,IL-10,TNF-a and IFN-γ in children with brain tumor were higher than those of control groups（P〈0. 01）. There were correlations between cytokines. The level of IL-8 in children with low grade of the tumor was higher than that in high grade of the tumor（P〈0.05）. CONCLUSIONS：The results impliy that the cytokines of IL-6, IL-8, TNF-a and IFN-γ may play role in the pathogenesis of pediatric brain tumors. There are interactions between cytokines. IL-8 can he used as a predictor of grade.

关 键 词：颅内肿瘤 白细胞介素 肿瘤坏死因子 干扰素 

分 类 号：R739.41[医药卫生—肿瘤]