荔枝果皮褐变过程中膜脂过氧化及相关酶活性变化的比较研究  

A Comparative Study on Changes in Membrane Lipid Super Oxidization and Related Enzyme Activities during Postharvest Pericarp Browning in Different Litchi Cultivars

作　　者：肖茜[1,2] 冯超[2,3] 刘保华[2,3] 李焕苓[2] 张新春[2] 杜中军[2] 王家保[2] 

机构地区：[1]海南大学园艺园林学院,海南海口570288 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [3]海南大学农学院,海南海口570228

出　　处：《热带作物学报》2011年第9期1653-1657,共5页Chinese Journal of Tropical Crops

基　　金：中央级科研院所基本业务费项目(No.2008hz1J018);现代农业产业技术体系专项资金(No.nycytx-32-06)

摘　　要：以紫娘喜、无核荔枝、小丁香等3个海南主栽荔枝品种为试材,研究了室温贮藏过程中果皮褐变与膜脂过氧化及有关酶活性变化的关系,结果表明,随着贮藏时间延长,3个品种果皮都表现出褐变指数上升,丙二醛(MDA)含量升高,质膜透性增大,抗坏血酸氧化酶(APX)活性下降,脂氧合酶(LOX)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性先升后降。但紫娘喜褐变最慢,无核荔枝褐变最快。耐贮的紫娘喜MDA含量、质膜透性及LOX活性较不耐贮的无核荔枝要低,GR活性较不耐贮藏的无核荔枝高。除GR活性外,无核荔枝其余指标变化幅度大于紫娘喜。不同品种果皮褐变进程差异与LOX活性变化、活性氧清除途径及质膜破坏进程差异有关。Postharvest changes in pericarp browning in relation to membrane lipid super oxidization and activities of related enzymes in pericarp were compared among cuhivars ＇Ziniangxi＇, ＇Wuhe＇and ＇Xiaodingxiang＇. The results showed that all the cultivars had increased browning index, malonaldehyde （MDA）content and relative membrane leak rate （RMLR）and a decreased ascorbic peroxidase （APX）activity in pericarp along with storage. Activities of catalase（CAT）and glutathione reductase（GR）increased 2-3 days after harvest and then decreased in all cultivars. ＇Wuhe＇turned brown most rapidly, while ＇Ziniangxi＇had the lowest browning rate. Correspondingly, ＇Ziniangxi＇ had lower MDA content, RMLR, LOX and CAT activities and a higher GR activity in the pericarp than ＇Wuhe＇. However, ＇Wuhe＇ had larger variations in the activities of all the tested enzymes but GR activities than ＇Ziniangxi＇.

关 键 词：荔枝 果皮褐变 膜脂过氧化 酶活性 

分 类 号：S667.1[农业科学—果树学]