间接免疫荧光法研究EB病毒感染类型与多发性硬化发生的关系  

The relationship between Epstein-Barr virus infection and multiple sclerosis detected by indirect immunofluorescence assay

作　　者：张善超[1] 刘磊[1] 宋兆慧[1] 谢琰臣[1] 王瑞金[1] 郭燕军[1] 易立[1] 脱厚珍[1] 王得新[1] 王佳伟[1] 

机构地区：[1]首都医科大学附属北京友谊医院神经内科,100050

出　　处：《中国神经免疫学和神经病学杂志》2011年第6期402-406,共5页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的用间接免疫荧光法研究EB病毒(EBV)不同感染类型与多发性硬化(MS)发生的关系。方法采用间接免疫荧光法检测20例MS患者和20例其他神经科疾病(OND)患者脑脊液(CSF)中抗EBV壳抗原(EBV-CA)IgG抗体、抗EBV-CA IgG抗体亲和力、抗EBV-CA IgM抗体、抗EBV早期抗原(EBV-EA)IgG抗体、抗EBV核心抗原(EBNA)抗体;根据CSF EBV抗体检测结果的组合情况,判断两组患者EBV急性感染(acute infection)、慢性感染(chronic infection)、原发性感染(primary infection)、感染后复发(recurrence of infection)和既往感染(past infection)的情况,并进行两组间比较。结果 (1)抗体水平:MS组抗EBV-CA IgG抗体低亲和力抗体阳性率(65%)、抗EBV-CA IgM抗体阳性率(75%)均明显高于OND组(分别为40%、25%,P<0.05)。两组之间抗EBV-CA IgG抗体、抗EBV-EA IgG抗体、抗EBNA抗体阳性率差异无统计学意义(P>0.05)。(2)感染类型比例:MS组EBV急性感染率(75%)明显高于OND组(40%,P<0.05),MS组既往感染率(20%)低于OND组(60%,P<0.05)。慢性感染、原发性感染、感染后复发率两组之间差异无统计学意义。结论 EBV急性感染可能与MS发病相关。Objective To investigate the infectious conditions of Epstein-Barr virus （EBV） in the patients with multiple sclerosis （MS） by indirect immunofluorescenee assay （IFA）. Methods In the twenty patients with MS and twenty patients with other neurological diseases （OND）, cerebrospinal fluid （CSF） was tested with IFA for anti-EBV eapsid antigen （EBV-CA） immunoglobulin G （IgG）, IgG affinity of anti-EBV capsid antigen, anti-EBV capsid antigen IgM, anti-EBV early antigen （EBV-EA） IgG and anti-EBV nuclear antigen （EBNA） IgG. According to the CSF antibody patterns, acute infection, chronic infection, primary infection, recurrence of infection and previous infection were compared between these two groups. Results There was no significant difference in anti-EBV-CA, anti-EBC-EA and anti-EBNA antigen IgG in CSF between MS patients and OND patients （P〈0.05）. The positive rate of low affinity of anti-EBV-CA IgG in MS patients was significantly higher than that in OND patients （65 % vs 40 %） （P〈0.05）. Significant difference in positive rate of anti-EBV-CA IgM was also found between MS and OND patients （75% vs 25 %） （P〈0.05）. 75% of MS patients were on acute stage of EBV infection compared with 40% of OND patients （P〈0.05）. Conclusions Acute infection of EBV is common in MS patients.

关 键 词：荧光抗体技术 间接 多发性硬化 疱疹病毒4型 人 

分 类 号：R744.51[医药卫生—神经病学与精神病学]