前体对多拉菌素生物合成的影响  

Effect of precursors on doramectin biosynthesis

作　　者：杨世红[1,2] 李能威[2] 郭伟群[2] 韩伟[2] 陈新[1] 张晓琳[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,武汉430023 [2]国家粮食局科学研究院,北京100037

出　　处：《中国抗生素杂志》2011年第11期829-832,共4页Chinese Journal of Antibiotics

基　　金：国家"863"计划(2006AA10A208-1)资助项目

摘　　要：目的通过研究前体对多拉菌素生物合成的影响,寻找提高多拉菌素发酵产量的有效方法。方法本文主要考察了不同前体对多拉菌素生物合成的作用,以及有效前体的最佳添加浓度和添加时间。结果丙酸钠是多拉菌素合成的最佳前体,在发酵96h时添加0.1%的丙酸钠,多拉菌素产量达139.89μg/mL,比对照组提高了38.2%。结论前体的供给能显著影响多拉菌素的生物合成,补加丙酸钠能有效提高多拉菌素的发酵产量。Objective Exploring an effective method of increasing doramectin productivity of Streptomyces avermitilis with the research of feeding of doramectin precursors. Methods The effects of different precursors on the biosynthesis of doramectin, the optimum concentration and feeding time of the effective precursor were studied. Result Sodium propionate was the optimal precursor for doramectin biosynthesis. By feeding of 0.1% sodium propionate into the fermentation broth at the 96th hour of cultivation, the doramectin production was 139.89μg/ mL,38.2% higher than that in the condition without any addition of precursor. Conclusion The feeding of precursors has significant influence on the doramectin biosynthesis and the addition of sodium propionate is effective in increasing doramectin production.

关 键 词：多拉菌素 阿维链霉菌 发酵 前体 

分 类 号：R978.15[医药卫生—药品]