高效降解甲醛菌株的分离鉴定及其特性  

Isolation and identification of bacterial strains efficient degrading formaldehyde and their degrading characteristics

作　　者：谢文娟[1,2] 王洁[1] 孙珮石[1] 邹平[1] 

机构地区：[1]云南大学环境与湖泊研究院,云南昆明650091 [2]云南大学生命科学学院,云南昆明650091

出　　处：《微生物学通报》2011年第11期1626-1631,共6页Microbiology China

基　　金：国家自然科学基金项目(No.50808154);国家863计划项目(No.2002AA649050)

摘　　要：首先对新分离的、能高效降解甲醛的两菌株A1和A2在形态学特征、生理生化特性及16S rDNA序列分析等方面进行了系统研究;随后通过测定在液体培养过程中甲醛浓度的变化,确定新分离菌株A1、A2降解溶液中甲醛的性能;最后利用菌株A1、A2分别进行生物填料塔的挂膜实验,确定其对甲醛气体的净化性能。结果表明:菌株A1属于假单胞菌属(Pseudomonas),菌株A2为鞘氨醇单胞菌属(Sphingomonas);当甲醛初始浓度<1 200 mg/L时,菌株A1、A2都能完全降解溶液中的甲醛,当甲醛浓度增高至1 600 mg/L时,菌株A1在48 h后的甲醛降解率为50%,菌株A2在104 h后的甲醛降解率为74.3%;菌株A1、A2对甲醛气体的净化效率均能达到99%以上,菌株A1的甲醛生化去除量能达到26.4 mg/(L.h),菌株A2的甲醛生化去除量可达20.6 mg/(L.h)。In this paper,two newly isolated bacterial strain A1 and strain A2,which were capable of degrading formaldehyde in high efficiency,were identified based on the results of standard morpho-logical identification,physiological and biochemical characters,16S rDNA sequence analysis.Their capability of degrading formaldehyde was investigated by determination of the formaldehyde concen-tration change in culture.The biological packed tower hung membrane experiment was carried to de-termine the purification performance of the two bacterial to formaldehyde gas.The results showed that： these two bacterial strain A1 and strain A2 were identified as Pseudomonas and Sphingomonas,respec-tively;when formaldehyde initial concentration was below 1 200 mg/L,it could be degraded by bacteria strain A1and strain A2 completely;when original formaldehyde concentration was up to 1 600 mg/L,50% of formaldehyde was consumed by strain A1 after 48 h,74.3% of formaldehyde was consumed by

关 键 词：甲醛 假单胞菌属 鞘氨醇单胞菌属 净化性能 

分 类 号：X172[环境科学与工程—环境科学]