拟细羽束梗孢发酵菌丝体中自由基清除活性成分分析  

Analysis of radical scavenging active constituents in the mycelia of Isaria gracilioides

作　　者：李康乐[1] 王小董[1] 张德龙[1] 陆瑞利[2] 胡丰林[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,安徽合肥230036 [2]安徽农业大学应用化学系,安徽合肥230036

出　　处：《微生物学通报》2011年第11期1740-1746,共7页Microbiology China

基　　金：国家自然科学基金项目(No.30871676);安徽省自然科学基金项目(No.11040606M69)

摘　　要：用二苯基苦基苯肼(DPPH)自由基法,首次对拟细羽束梗孢(Isaria gracilioides RCEF3 279)菌丝体的不同溶剂提取物进行了自由基清除活性的定性定量分析,发现其甲醇提取物具有较强的清除自由基活性,当菌丝浓度为10 g/L时,其甲醇提取物的自由基清除率达到了92.4%±0.3%。DPPH自显影-薄层色谱分析结果显示有4个具自由基清除活性主要成分。高分辨液质联用和活性联合分析发现,提取物中4个具自由基清除活性成分的可能分子式分别为C15H16NO3(1)、C32H40N2O18(2)、C30H55N5O5(3)和C17H14N2O2(4)。经天然产物数据库查询发现,化合物3和4可能分别为Viscumamide和Dehydrocyclopepti,这两个化合物在虫生真菌代谢产物中属首次发现。数据库查询没有发现与分子式C15H16NO3和C32H40N2O18匹配的结构式,因此化合物1和2可能为新化合物。A DPPH-microplate assay was employed for quantitative analysis of the free radical scav-enging activity of the extracts extracted with different solvents from mycelia of Isaria gracilioides.The results showed that the methanol extract had the strongest free radical scavenging activity.At the con-centration of 10 mg mycelium per milliliter methanol,the radical scavenging rate of the extract reached 92.4%±0.3%.The results of DPPH-TLC assays showed that the methanol extract contained four main free radical scavenging components.The combined LC-DAD-HRMS and DPPH assay revealed that the molecular formulas of the four free radical scavengers were possibly C15H16NO3（1）,C32H40N2O18（2）,C30H55N5O5（3） and C17H14N2O2（4）.Natural products database query showed that compounds 3 and 4were possibly viscumamide and dehydrocyclopeptin,respectively,and compounds 1 and 2 were novel molecules.Viscumamide and dehydrocyclopeptin were detected from entomogenous fungi for the first time.

关 键 词：拟细羽束梗孢 清除自由基 活性测定 液质联用 二苯基苦基苯肼(DPPH) 

分 类 号：Q936[生物学—微生物学]