蜂蜜增强阿昔洛韦对单纯疱疹病毒性角膜炎模型兔药效的最佳浓度实验研究  

Study on the Optimal Concentration of Honey to Enhance the Effect of Acyclovir on Herpes Simplex Virus Keratitis Model Rabbits

作　　者：何群[1] 王适[2] 张湘晖[3] 伍参荣[1] 姜宇[1] 赵碧清[1] 

机构地区：[1]湖南中医药大学中药现代化实验室,长沙市410208 [2]湖南中医药大学第二临床学院,长沙市410005 [3]湖南中医药大学第一临床学院,长沙市410007

出　　处：《中国药房》2011年第45期4238-4240,共3页China Pharmacy

基　　金：湖南省科学技术厅科技计划(2008FJ3068)

摘　　要：目的:探索蜂蜜增强阿昔洛韦(ACV)对单纯疱疹病毒性角膜炎(HSK)模型兔药效的最佳浓度,为2药联用提供依据。方法:对兔角膜采用上皮划痕法接种单纯疱疹病毒Ⅰ型病毒株建立HSK模型,将模型兔随机分为7组,每组4只(8只眼),分别为:Ⅰ～Ⅳ组(蜂蜜与3%ACV混合凝胶,其中蜂蜜含量分别为0、3%、5%、7%)、Ⅴ组(只建模)、Ⅵ组(3%ACV眼膏)、Ⅶ组(空白基质),每日涂相应眼药3次,每次给药0.1g,间隔4h,连续给药12d。用裂隙灯观察各组给药第3、6、9、12天(次日给药前观察)情况并进行药效评分,再对药效评分进行等级一致性检验,找出药效最优组别。结果:每组各时间段药效评分结果不同,但从综合药效评分排序可知:Ⅲ组>Ⅳ组>Ⅱ组>Ⅵ组>Ⅰ组≈Ⅶ组≈Ⅴ组,等级有一致性的倾向,以含5%蜂蜜与3%ACV混合凝胶药效最强,优于其他组。结论:蜂蜜增强3%ACV对HSK药效的最佳浓度为5%。OBJECTIVE： To explore the optimal concentration of honey to enhance the effect of acyclovir （ACV） on the rabbits with herpes simplex virus keratitis （HSK）, and to provide reference for drug combination. METHODS： Rabbit cornea were vaccinated against type I herpes simplex strain by epithelium scratch method to establish HSK model. Model rabbits were randomly divided into 7 groups with 4 rabbits （8 eyes） in each group： i.e. groups I -IV （gel of honey mixed with 3% ACV, containing 0, 3%, 5%, 7% honey） ; group V （model established only） ; group VI （3% acyclovir eye ointment） ; groups VII （blank matrix）. They were given relevant medicine 3 times a day, 0.1 g each time every 4 hour for 12 days. Slitlampmicroscope was used to observe therapeutic efficacy on 3rd, 6th, 9th, 12th day （before administration the next day）. Level consistency test of therapeutic efficacy was conducted to find out optimal therapeutic efficacy. RESULTS： The pesticide effects of each group were different at different time in descending sequence： group III 〉grouplV 〉group 1I 〉group VI 〉group I ≈groupVII vgroup V, towards to consistency. Curative effect of the mixed gel of 5 % honey and 3 % ACV was the best, better than other groups. CONCLUSION： The optimal concentration of honey combined with 3% ACV is 5% in the treatment of HSK.

关 键 词：蜂蜜 阿昔洛韦 单纯疱疹病毒性角膜炎 兔 最佳浓度 

分 类 号：R965[医药卫生—药理学]