胰岛素样生长因子和血管内皮生长因子在宫颈病变中的表达及其意义  

Expression and significance of IGF-Ⅱ and VEGF in cervical lesions

作　　者：刘萍[1] 侯小赛[1] 王辉[1] 

机构地区：[1]内蒙古医学院第三附属医院妇产科,内蒙古包头014010

出　　处：《中国现代医学杂志》2011年第29期3643-3646,共4页China Journal of Modern Medicine

摘　　要：目的通过检测宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)和宫颈鳞癌(cervical squa-mous cell cancinomas)中胰岛素样生长因子Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)及血管内皮生长因子(vascular endothelial cell growth factor,VEGF)的表达情况,分析两者的相关性。方法应用免疫组织化学SP法检测37例宫颈鳞癌、30例CINⅢ、31例CINⅠ、Ⅱ、30例正常宫颈组织的石蜡切片中IGF-Ⅱ和VEGF的表达情况。结果①IGF-Ⅱ在正常宫颈组织、CINⅠ、Ⅱ、CINⅢ及宫颈鳞癌中的阳性表达率分别为10.00%、38.71%、73.33%和94.59%,差异有统计学意义(P<0.05)。②VEGF蛋白在正常宫颈组织CINⅠ、Ⅱ、CINⅢ及宫颈鳞癌中的阳性表达率分别为6.67%、29.03%、66.67%和83.8%,差异有统计学意义(P<0.05)。③经Spearman等级相关分析显示,宫颈病变中IGF-Ⅱ和VEGF阳性表达呈正相关(rs=0.915,P=0.000,P<0.05)。结论①随着宫颈病变由CINⅠ、Ⅱ至CINⅢ、宫颈鳞癌的进展,IGF-Ⅱ和VEGF蛋白的阳性表达率均呈上升趋势。②宫颈病变中IGF-Ⅱ与VEGF蛋白的阳性表达成正相关。[ Objective] To investigate the significance of insulin-like growth factor-Ⅱ （IGF-Ⅱ） and its correlation with angiogenesis in precancerosis and cervical squamous cell carcinomas by detecting the expression of IGF-Ⅱ and vascular endothelia cell growth factor （VEGF） in cervical intraepithelial neoplasia （CIN） and cervical squamous cell carcinomas. [Methods] Immunohistochemical S-P method were used to detect the expression of IGF-Ⅱ and VEGF in paraffin-embedded specimens of 37 cases cervical squamous cell carcino- mas, 30 cases CIN Ⅲ, 31 cases of CIN I -Ⅱ and 30 cases of normal cervical tissue. [Results] The positive percentage of expressions of IGF- Ⅱ in normal cervical tissue, CIN I -Ⅱ, CIN Ⅲ and cervical squamous cell cancer were 10%, 38.7%, respectively, 73.33% and 94.59%, （P〈0.05）. The positive percentage of expressions of VEGF in normal cervical tissue, CIN I -Ⅱ, CIN lit and cervical squamous cell cancer were 6.67%, 29.03%, 66.67% and 83.80%, （P〈0.05）. The expression of IGF-Ⅱ had positive correlation with the VEGF by Spearman rank correlation test （r=0.965, P〈0.05）. [Conclusion] The positive expression rate of protein of IGF-Ⅱ and VEGF showed tendency of ascensus with the progression of cervical lesions. The expression of IGF-Ⅱ had positive correlation with the VEGF in cervical lesions.

关 键 词：宫颈鳞癌 宫颈上皮内瘤变 IGF-Ⅱ VEGF 免疫组织化学 

分 类 号：R737-33[医药卫生—肿瘤]