荧光法测定维药刺糖中氨基酸的含量  

Determination of Amino Acid in Saccharum Alhagi from Uygur Medicine by Fluorimetry

作　　者：李改茹[1] 常军民[1] 王岩[1] 吴晶[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐市新医路393号830054

出　　处：《光谱实验室》2011年第6期2965-2968,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：新疆维吾尔自治区高校科研计划(XJEDU2009S49)

摘　　要：建立维药刺糖中氨基酸含量测定的荧光分析法。刺糖经蒸馏水超声提取,氨基酸用0.1%邻苯二甲醛衍生化后用荧光分析法测其氨基酸含量。测定的线性范围为:3.0952—15.5071μmol.L-1(r=0.9990),检出限为9.70×10-7mol.L-1,平均回收率为93.6%—99.7%,RSD为1.9%—2.5%。该法灵敏度高,且简便、快捷,适用于天然药物中氨基酸的含量测定。The method for the determination of the content of amino acid in Saccharum alhagi from uygur medicine was established by fluorimetry.Amino acid was extracted from Saccharum alhagi by ultrasound with distilled water,and determined by fluorimetry after derived by 0.1% orthophthalaldehyde（OPA）.The linear range was 3.0952—15.5071μmol·L-1（r=0.9990）.The detection limit was 9.70×10-7mol·L-1.The average recoveries of samples were 93.6%—99.7% with RSD in the range of 1.9%—2.5%.The method is simple,rapid and high sensitivity,which can be used for the determination of the contents of amino acids in natural drugs.

关 键 词：刺糖 氨基酸 荧光分析法 

分 类 号：O657.32[理学—分析化学]