人附红细胞体感染实验诊断方法的建立  

Establishment of diagnostic methods to detect eperythrozoon in human

作　　者：王剑飚[1] 樊绮诗[1] 倪麟[2] 倪培华[2] 姚玉峰[3] 

机构地区：[1]上海交通大学医学院附属瑞金医院检验科,上海200025 [2]上海交通大学医学院检验系,上海200025 [3]上海交通大学医学院病原生物学教研室,上海200025

出　　处：《检验医学》2011年第11期784-790,共7页Laboratory Medicine

摘　　要：目的比较瑞氏-吉姆萨染色、吖啶橙荧光染色以及聚合酶链反应(PCR)对人附红细胞体的检出率,探索最可靠的检测人附红细胞体病的方法。方法选取体检健康人50名,同时从白血病及肾移植患者中分别选取50例,使用瑞氏-吉姆萨染色法以及吖啶橙荧光染色法检测附红体的感染率,同时选取1例2种方法检测结果均为强阳性的标本,根据GenBank中已收录的猪、牛、羊、猫、鼠等5种附红细胞体的16S rRNA基因序列设计一对特异性引物,初步建立人附红体病的分子诊断方法。结果体检健康人群中瑞氏-吉姆萨染色法以及吖啶橙荧光染色法检出率分别为32%和24%,白血病组的检出率分别为94%和62%,肾移植组的检出率分别为88%和68%,同时初步建立了人附红细胞体病的分子诊断方法,但需进一步研究将其完善。结论吖啶橙荧光染色法最适合临床作为人附红细胞体病的临床检测方法。Objective To compare the detection rates of the Wright-Giemsa staining method, acridine staining method and polymerase chain reaction （PCR） for detecting eperythrozoon in human, in order to search for a reliable method to detect eperythrozoon in human. Methods 50 healthy subjects, 50 leukemia patients and 50 kidney transplantation patients were enrolled in this study. The infection rates were detected by Wright-Giemsa staining method and acridine staining method. The samples with the results＇ both positive by the 2 methods were selected. A pair of primer specific to eperythrozoon in human was designed according to the 16S rRNA gene sequence of Eperythrozoon suis, Eperythrozoon wenyonii, Eperythrozoon ovis, Eperythrozoon haemofelis and Eperythrozoon coccoides from GenBank, and a diagnostic method was initially established to detect human eperythrozoonosis. Results The detection rates in healthy subject group, leukemia group and kidney transplantation group were 32%, 94% and 88% by Wright-Giemsa staining method, and the detection rates were 24% , 62% and 68% by acridine staining method. The diagnostic method was initially established to detect human eperythrozoonosis, and it should be improved further. Conclusions The acridine staining method is suitable for the clinical detection of eperythrozoon in human.

关 键 词：附红细胞体 瑞氏-吉姆萨染色 吖啶橙 PCR 16S RRNA 

分 类 号：R446.11[医药卫生—诊断学]