子宫内膜癌Ⅰ型和Ⅱ型miRNA表达谱差异的分析  

Expression profiles of miRNA in typeⅠandⅡendometrial carcinoma

作　　者：薛纪森[1] 张乾[1] 李晓琳[1] 胡燕[1] 杨孝军[1] 颜笑健[1] 郑飞云[1] 

机构地区：[1]温州医学院附属第一医院妇科,325000

出　　处：《实用医学杂志》2011年第22期4051-4054,共4页The Journal of Practical Medicine

基　　金：浙江省自然科学基金一般项目(编号:Y2090699)

摘　　要：目的:分析Ⅰ型和Ⅱ型子宫内膜癌及良性子宫内膜组织间MiRNA表达谱差异。方法:收集Ⅰ型和Ⅱ型子宫内膜癌患者内膜及良性患者内膜组织,采用mirVana RNA isolation kit提取总RNA,利用芯片检测miRNA表达谱差异。结果:Ⅰ型和Ⅱ型子宫内膜癌及良性内膜组织间均有差异表达的miRNA,其中Ⅰ型内膜癌与良性内膜组织间存在59个表达差异miRNAs,Ⅱ型内膜癌与良性内膜组织间存在13个差异表达miRNAs,Ⅰ型与Ⅱ型子宫内膜癌间存在30个差异表达miRNAs。结论:Ⅰ型和Ⅱ型子宫内膜癌间miRNA的表达差异可能与不同类型子宫内膜癌的发生发展相关,可为进一步研究子宫内膜癌发生的分子机制提供依据。Objective To study miRNA expression patterns in type Ⅰ and Ⅱ endometrial carcinoma （EC） and benign endometrium. Methods Fresh-fl＇ozen samples of type Ⅰ and Ⅱ endometrial carcinoma and benign endometrial samples were collected.Total RNA was extracted from samples using the mirVana RNA isolation kit. MiRNA expression profiles were evaluated by microarray analysis. Results Distinct miRNA signatures in tumor versus benign samples were detected. 59 distinct miRNAs were identified in type Ⅰ ECs compared with benign endometrial samples, whereas 13 distinct miRNAs were identified in type Ⅱ ECs. Comparison of miRNA expression profiles between type Ⅰ and type Ⅱ ECs revealed 30 different miRNAs. Conclusion Different expression profiles of miRNA between type Ⅰ and type Ⅱ ECs may provide insights into their roles in the pathogenesis and progression of EC.The study of miRNA contributes to elucidate the molecular mechanism of endometrial carcinoma.

关 键 词：子宫内膜肿瘤 MICRORNA MIRNA芯片 

分 类 号：R737.33[医药卫生—肿瘤]