叶下珠复方Ⅱ号对小鼠H22肝癌移植瘤生长的抑制作用  

Inhibitory action of compound phyllanthus urinaria(CPUⅡ) on the growth of transplant hepatoma(H22) in mice

作　　者：李常青[1] 申建柯[1] 李小翚[2] 

机构地区：[1]广州中医药大学热带医学研究所,广东广州510405 [2]广州中医药大学中药学院,广东广州510405

出　　处：《中国热带医学》2011年第10期1182-1183,1189,共3页China Tropical Medicine

基　　金：广东省科技计划资助项目(No.2010B030700026)

摘　　要：目的观察叶下珠复方Ⅱ号对小鼠H22肝癌移植瘤生长的抑制作用,并探讨其可能的作用机制。方法取昆明小鼠40只,建立小鼠H22肝癌移植瘤模型,分为模型组、叶下珠复方Ⅱ号高剂量组(37.5g/kg)、低剂量组(18.75g/kg)和环磷酰胺(20mg/kg)治疗组。叶下珠复方Ⅱ号高、低剂量组均灌胃给药,环磷酰胺组采用腹腔注射,每日1次,连续8d。末次给药24h后,测定瘤体质量,计算抑瘤率;检测血清IL-2和TNF-а含量。结果叶下珠复方Ⅱ号高、低剂量组瘤重明显低于模型组,差异均有显著性意义(P<0.01),但与环磷酰胺组比较,差异均无显著性意义(P>0.05)。叶下珠复方Ⅱ号高、低剂量组的抑瘤率分别为67%和58%,与环磷酰胺组接近。叶下珠复方Ⅱ号高剂量组血清IL-2水平明显高于模型组(P<0.05)。叶下珠复方Ⅱ号高、低剂量组血清TNF-а水平明显低于模型组,差异有显著性意义(P<0.05)。结论叶下珠复方Ⅱ号有较好的抑制小鼠H22肝癌移植瘤生长作用,作用机制与调节免疫,升高IL-2水平和抑制TNF-а水平有关。Objective To observe the inhibitory effects of CPUⅡ on the growth of transplant Hepatoma 22（H22） in mice.Methods Forty NIH mice were established for transplation tumour model of H22,then they were randomly divided into four groups： model group（group A）,high and low dosage group of CPU Ⅱ（group B,group C）,and cyclophosphamidum as the positive control（group D）.Mice in group B and group C were administrated orally with CPU Ⅱ at dosage of 37.5 g/kg and 18.75 g/kg daily respectively.Mice in group D were injected with cyclophosphamidum intraperitoneal at dosage of 20mg/kg daily.Eith days after treatment,the tumour-inhibition rate were calculated according to the tumour mass weight in mice,the serum levels of IL-2 and TNF-а were determined.Results It showed that tumour mass weight in group B and group C were obviously lower than that of group A（P0.01）,but without significant difference comparing with that of group D（P0.05）.The tumour-inhibition rate in group B and group C were 67% and 58%,resepectively,closing the group D.The serum IL-2 level in group B were obviously higher than that of group A（P0.05）.The serum of TNF-a in group B and group C were obviously lower than that of group A（P0.05）.Conclusion CPUⅡ showed good effect of inhibiting transplanted tumour growth on H22 tumour-bearing mice.The mechanism is related to immune regulation through decreasing TNF-a level and increasing IL-2 level.

关 键 词：叶下珠复方Ⅱ号 小鼠 H22肝癌移植瘤 抑制 

分 类 号：R737.7[医药卫生—肿瘤]