家蝇蛆粉的临床前致畸性研究  

Study on the preclinical teratogenicity of Musca domestica larvae powder

作　　者：黎运西[1] 朱家勇 金小宝 曾爱华[3] 褚夫江 

机构地区：[1]南方医科大学附属顺德第一人民医院检验科,广东佛山528300 [2]广东省生物活性物质研究重点实验室,广东广州528300 [3]广东药学院药科学院,广东广州510000

出　　处：《中国热带医学》2011年第10期1259-1260,共2页China Tropical Medicine

基　　金：广东省教育部产学研结合项目(No.2008B090500191);广东省产业技术研究与开发项目(No.2008B080703065)

摘　　要：目的探讨家蝇蛆粉对大鼠的致畸性,以评价其安全性。方法家蝇蛆粉按比例定量添加到普通饲料原料中混匀,干燥,做成高、中、低剂量家蝇蛆粉饲料。将性成熟的98只SD大鼠按雌雄2:1的比例同笼交配。取64只孕鼠平均分为四组:家蝇蛆粉饲料低剂量组;家蝇蛆粉饲料中剂量组;家蝇蛆粉饲料高剂量组;普通饲料对照组。于受孕后称量体重增长情况。最后脱颈椎处死孕鼠,称子宫重量,并进行胎鼠检查。结果家蝇蛆粉饲料高、中、低剂量组与对照组相比,雌鼠体重增长未见显著差异,子宫重量未见显著差异,无吸收胎、早死胎、晚死胎;各组间雄性与雌性胎鼠数均未见显著差异;各组间雄性与雌性胎鼠的外观、体重、体长均未见显著差异。各组间胎鼠骨骼和胎鼠内脏均未见显著差异。结论家蝇蛆粉对SD大鼠未见致畸性。Objective This paper evaluated teratogenicity on mice of powder extract from Musca domestica larvae powder（MDLP） to evaluate the safety of it.Methods MDLP was mixed and added to animal feeds for preparation of high dose,moderate dose and low dose of MDLP animal feeds.In each cage 2 female eugamic rats and 1 male eugamic rat were put together for mating.After conception,the 64 pregnant rats were divided into the following group： high dose of MDLP animal feeds group,middle dose of MDLP animal feeds group,low dose of MDLP animal feeds group,common animal feeds group.growth information of body weight of the above 4 group pregnant rats were weighed.At the endpoint,pregnant rats were sacrificed and their uteruses weighed and the fetuses examined.Results The body weight and uteruses weight of pregnant rats,fetus＇appearance,fetus＇ sex,fetus＇body weight,fetus＇stem length,fetus＇ bone and fetus＇ internal organs specimen of mice in high,moderate and low dose groups showed no significantly difference compared with that of the negative group.No absorpted foetus,earlier died foetus before delivery,later died foetus before delivery for three different dose groups.Conclusion There no teratogenic toxicity of MDLP to SD mice was observed.

关 键 词：家蝇蛆粉 SD大鼠 致畸性 

分 类 号：R-331[医药卫生]