硝苯地平对急性铁负载大鼠黑质DA能神经元的作用  

NEUROPROTECTIVE EFFECT OF NIFEDIPINE ON THE DOPAMINERGIC NEURONS IN SUBSTANTIA NIGRA OF ACUTE IRON-OVERLOADED RATS

作　　者：王荣[1] 马泽刚[1] 谢俊霞[1] 

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东青岛266071

出　　处：《青岛大学医学院学报》2011年第5期377-378,381,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金项目(30800353);青岛市科技局项目(09-1-3-80-jch);青岛市科技局项目(09-1-1-29-nsh)

摘　　要：目的探讨L型电压门控型Ca2+通道阻断剂硝苯地平对急性铁负载大鼠黑质多巴胺(DA)能神经元的作用。方法雄性Wistar大鼠随机分为对照组、急性铁负载组以及硝苯地平干预组。应用逆转录-聚合酶链反应、铁含量测定等技术,检测黑质酪氨酸羟化酶(TH)mRNA的表达水平及铁含量。结果急性铁负载组大鼠黑质TH mRNA的表达水平较对照组明显降低,铁含量较对照组明显增高,硝苯地平预处理可逆转上述改变(F=100.293、56.202,P<0.01)。结论硝苯地平对急性铁负载大鼠黑质DA能神经元具有保护作用。Objective To investigate the effect of nifedipine（a blocker of L-type calcium channels） on dopaminergic neurons in the substantia nigra（SN） of acute iron-overloaded rats. Methods Male Wistar rats were randomly divided into control group,iron-overloaded group,iron-overloaded with nifedipine treatment group.The expression levels of tyrosine hydroxylase（TH） gene in SN were detected by reverse transcription-polymerase chain reaction,and the iron content of SN were determined by iron concentration assay. Results Compared with the control group,the expression of TH mRNA decreased,and the iron content increased in the acute iron-overloaded SN of rats.Pretreatment with nifedipine in the acute iron-overloaded rats could partly restore the above changes（F=100.293,56.202;P0.01）. Conclusion Nifedipine may inhibit acute iron-overload induced dopamine neuron damage in the iron-overloaded rats.

关 键 词：硝苯地平 铁 黑质 多巴胺 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]