宫颈癌组织细胞分化抑制因子-1表达及其意义  

EXPRESSION OF CELL DIFFERENTIATION INHIBITING FACTOR-1 IN TISSUE OF CERVICAL CANCER

作　　者：渠力平[1,2] 于小玲[2] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院妇产科,山东烟台264000 [2]青岛大学医学院病理生理学教研室

出　　处：《齐鲁医学杂志》2011年第6期486-488,共3页Medical Journal of Qilu

摘　　要：目的了解细胞分化抑制因子-1(Id-1)mRNA和蛋白在人宫颈癌组织中的表达及其意义。方法分别应用荧光实时定量逆转录-聚合酶链反应和免疫组织化学方法,检测Id-1 mRNA和蛋白在6例人正常宫颈、6例宫颈上皮内瘤样变(CIN)和24例宫颈癌组织中的相对表达定量,分析Id-1表达与宫颈癌病理参数间的相关性。结果 Id-1蛋白在正常宫颈组织未见阳性表达,在CIN组织中有少量表达,在宫颈浸润癌组织中呈明显的阳性表达,宫颈浸润癌组织Id-1蛋白阳性面积百分比和阳性染色吸光度均显著高于CIN组织(t=7.80、3.15,P<0.05)。正常宫颈组织、CIN组织和宫颈癌组织Id-1 mRNA相对定量依次增高,差异有显著性(F=3 030.30,q=17.78、95.04,P<0.01)。宫颈癌组织中Id-1 mRNA和蛋白表达呈正相关(r=0.89,P<0.01)。Id-1表达和宫颈癌组织分级及FIGO分期呈正相关(r=0.67、0.58,P<0.01、0.05)。结论检测Id-1表达有助于宫颈癌的辅助诊断。Objective To study the expressions of cell differentiation inhibiting factor-1（Id-1） mRNA and protein in human cervical cancer and its significance.Methods The quantity of Id-1 mRNA and protein were detected by real time RT-PCR and immunohistochemical method,respectively,in six samples of normal cervix,six of cervical intraepithelial neoplasia（CIN） and 24 of cervical cancer.The relationship between Id-1 and some clinical parameters was analyzed.Results No positive expressions of Id-1 mRNA and protein were seen in tissue of normal cervix;few expressions in CIN;obvious expressions in cervical cancer.The percentage of expression area and density of the cancer tissue were higher than that of CIN,the difference being significant（t=7.80,3.15;P〈0.05）.The relative ration of Id-1 mRNA increased in order of normal tissue,CIN tissue and cancer tissue,with a significant difference between them（F=3 030.30;q=17.78,95.04;P〈0.01）.In tissue of cervical cancer,Id-1 mRNA was positively correlated with protein（r=0.89,P〈0.01）,and with the grading and FIGO staging of the cancer tissue（r=0.67,0.58;P〈0.01,0.05）.Conclusion A detection of Id-1 is conducive to auxiliary diagnosis of cervical cancer.

关 键 词：宫颈肿瘤 分化抑制因子-1 逆转录聚合酶链反应 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]