肝癌细胞p15基因CpG岛甲基化研究  

Study on p15 gene methylation status of CpG island in hepatocellular carcinomas

作　　者：王松[1] 许诚[1] 舒丹[1] 何清[1] 叶涛生[1] 李丽雄[1] 周陶友[2] 陈守春 

机构地区：[1]深圳市东湖医院,深圳518020 [2]四川大学华西医院,成都610041 [3]成都地奥制药集团,成都610041

出　　处：《中国肝脏病杂志（电子版）》2008年第1期10-12,共3页Chinese Journal of Liver Diseases：Electronic Version

基　　金：深圳市卫生科技计划(200404159)

摘　　要：目的通过检测肝癌细胞株HepG2的抑癌基因p15基因启动子区CpG岛的甲基化状态,探讨其与肿瘤发生的可能相关性。方法应用甲基化特异性PCR(MSP)技术,对人肝癌细胞株HepG2的p15基因启动子区域CpG岛的甲基化状态进行检测,以人淋巴瘤细胞株Raji为阳性对照,以正常人外周血单核细胞(PBMC)和肝细胞为阴性对照。结果肝癌细胞HepG2中p15基因启动子区域CpG岛甲基化和非甲基化检测均呈阳性,正常人外周血单核细胞和肝细胞甲基化检测阴性。结论肝癌细胞株HepG2抑癌基因p15基因CpG岛存在高度甲基化,可能与肝癌的发生相关。Objective To investigate methylation status of CpG island on p15 gene promoter domain and its relationship with hepatocellular carcinoma.Methods Methylation status of CpG island on p15 gene promoter domain in HepG2 cells was detected by methylation specific PCR and Raji cells were taken for positive control while normal PBMC and hepatocytes as negative control.Results Both methylation and non-methylation status of CpG island on p15 gene promoter domain in HepG2 cells were detected,but methylation in negative controls were not detected.Conclusions Methylation status of CpG island on p15 gene promoter domain exist in HepG2 cells which may associate with hepatocellular carcinoma.

关 键 词：肝癌 DNA甲基化 P15基因 

分 类 号：R735.7[医药卫生—肿瘤]