Effects of Different Culture Condition in vitro on Growth of Cashmere Goat Primary Hair Follicles  

不同条件对绒山羊体外培养初级毛囊生长的影响(英文)

作　　者：李蘅[1] 李金泉[2] 曹贵方[1] 张文广[2] 

机构地区：[1]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《Agricultural Science & Technology》2008年第5期43-46,共4页农业科学与技术（英文版）

基　　金：Supported by Regional Key Project of National Natural Science Foundation of China(39969002);Key Project of National Natural Science Foun-dation of Inner Mongolia Autonomous Region(200408020401)~~

摘　　要：[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium.[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium.

关 键 词：CASHMERE GOAT PRIMARY HAIR follicle Culture in VITRO 

分 类 号：S827[农业科学—畜牧学]