褪黑素受体1B基因rs10830963位点多态性与妊娠期糖尿病遗传易感性的关系  

Association of genetic variant rs10830963 of melatonin receptor 1B gene in women with gestational diabetes mellitus

作　　者：邓志凤 舒青青 陈友好 向美华 李笑 吴胜兰 张敏飞 宋伟 

机构地区：[1]浙江省衢州市妇幼保健院内科,324000

出　　处：《中华围产医学杂志》2011年第11期666-669,共4页Chinese Journal of Perinatal Medicine

基　　金：浙江省衢州市科技项目（20101091）

摘　　要：目的探讨褪黑素受体1B（melatonin receptor1B，MTNR1B）基因rs10830963位点单核苷酸多态性与妊娠期糖尿病（gestational diabetes mellitus，GDM）遗传易感性的关系。方法选择来自浙江省衢州市妇幼保健院的GDM孕妇87例（GDM组）和同期正常妊娠妇女91例（对照组），应用PCR扩增和DNA测序技术确定所有研究对象MTNR1B基因rs10830963位点等位基因和基因型频率分布，并比较2组之间rs10830963位点等位基因和基因型频率的差异。此外，利用Logistic回归分析判断风险等位基因与空腹血糖和糖化血红蛋白（glycosylated hemoglobinA1c，HbAlc）的关系。结果GDM组rsl0830963位点G等位基因频率和GG基因型频率均明显高于对照组，G等位基因携带者和GG基因型妇女发生GDM危险性分别是C等位基因和CC基因型的1．53倍（OR=1．53，95％CI：1．005～2．324，P=0．047）和2．16倍（OR=2．16，95％CI：1．052～4．434，P=0．034）。利用Logistic回归校正年龄、孕前体重指数（body mass index，BMI）和糖尿病家族史等因素之后，GG基因型妇女发生GDM的危险性仍然显著增加（OR=2．07，95％CI：1．048～4．372，P=0．022）。多元线性回归分析显示风险等位基因G与空腹血糖（0．068mmol／L，P=0．015）水平及HbAlc（0．073％，P=0．028）水平呈显著正相关。结论MTNR1B基因rs10830963位点单核苷酸多态性和GDM遗传易感性有关。rs10830963位点G等位基因为GDM发生的遗传风险因子。Objective To investigate whether the genetic variant rs10830963 of melatonin receptor 1B （MTNR1B） gene is associated with increased risk for gestational diabetes mellitus （GDM）. Methods Eighty-seven GDM subjects （GDM group） and 91 normal pregnant women （control group） were randomly recruited form Women and Children＇s Hospital of Quzhou, Zhejiang Province, China. The allele and genotype frequencies of the rs10830963 in MTNR1B gene were determined in all participants with PCR amplification and DNA sequencing. The allele and genotype frequencies of rs10830963 were compared to determine their differences between GDM subjects and normal controls. In addition, multiple linear regression was conducted to investigate the association patterns of the risk allele with fasting glucose and HbAlc levels. Results Both GG genotype and G allele frequencies of the rs10830963 loci in the GDM group were significantly higher than those in the controls, and women with G allele and GG genotype were associated with increased GDM risk （OR= 1.53, 95 % CI： 1. 005-2. 324, P= 0. 047 and OR= 2.16, 95 % CI： 1. 052 4. 434, P = 0. 034 respectively）. After adjusting for age, body mass index before pregnancy, and family history of diabetes mellitus, women carrying GG genotype still had a higher GDM risk （OR = 2.07, 95% CI= 1. 048-4. 372, P = 0. 022）. Multiple linear regression showed that the rs10830963 G allele was positively correlated with higher levels of fasting glucose （0. 068 mmol/L, P= 0. 015） and HbAlc （0. 073%, P=0. 028）. Conclusions Genetic variant rs10830963 in MTNRIB gene may contribute to the susceptibility to GDM in Chinese population and the rs10830963 G allele is a risk factor for the GDM susceptibility.

关 键 词：糖尿病 妊娠 受体 褪黑激素 MT2 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R714.25[医药卫生—妇产科学]