Antioxidase System of a Rice Mutant with Low Chlorophyll b  

低叶绿素b水稻突变体的抗氧化酶系统研究(英文)

作　　者：谢雅晶[1] 陈海燕[1] 张荣铣[2] 张炜[1] 

机构地区：[1]南京农业大学生命科学学院生物化学与分子生物学系,江苏南京210095 [2]南京农业大学生命科学学院植物科学系,江苏南京210095

出　　处：《Agricultural Science & Technology》2008年第3期6-10,30,共6页农业科学与技术（英文版）

基　　金：国家自然科学基金(30400030);江苏省创新人才基金资助项目~~

摘　　要：[Objective] The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] A rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparatively research their peroxide (H2O2) contents, the activity and isozymes of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in chloroplast.[Result] Compared with the wild type, there were many kinds of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant, and the activity of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant was also correspondingly higher. Under intense light condition, the H2O2 content of chloroplast in mutant was less than that in the wild type. [Conclusion] The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane, which is an important reason for higher photosystem Ⅱ (PS II) stability of this mutant.[Objective] The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] A rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparatively research their peroxide (H2O2) contents, the activity and isozymes of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in chloroplast.[Result] Compared with the wild type, there were many kinds of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant, and the activity of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant was also correspondingly higher. Under intense light condition, the H2O2 content of chloroplast in mutant was less than that in the wild type. [Conclusion] The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane, which is an important reason for higher photosystem Ⅱ (PS II) stability of this mutant.

关 键 词：MUTANT with LOW CHLOROPHYLL b ORYZA SATIVA Antioxidant enzyme ISOZYME pattern 

分 类 号：S511[农业科学—作物学]