White-rot Fungus Growth Conditions and Its Metabolic Kinetic Models  

白腐真菌生长条件及其代谢动力学模型(英文)

作　　者：梁峙[1,2] 韩宝平[1] 

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221116 [2]徐州工程学院环境工程系,江苏徐州221008

出　　处：《Agricultural Science & Technology》2008年第6期1-9,共9页农业科学与技术（英文版）

基　　金：Supported by National Natural Sciences Foundation of China(40373044);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(05KJD610209)~~

摘　　要：To investigate the growth conditions of white-rot fungus and simulate its metabolism kinetic models, the rules how the factors such as biomass, culture fluid, pH value, glucose consumption and exopolysaccharides generation, etc., changed during the batch culture process of white-rot fungi by using an air-lift fermenter, as well as metabolic kinetics of white-rot fungi were studied. Based on Logistic equation, Luedeking-Piret equation and experimental data, the correlation model parameters of mycelia biomass, glucose consumption and exopolysaccharide generation were obtained and found to be change with time in metabolism process. Detailedly, μm=0.071 8 h-1,α= 0.831 8 g/(g·h), β= 0.002 g/(g·h), b1=0.016 3 g/(g·h) and b2=3.023 3 g/(g·h). Hence the mycelial growth kinetic model, exopolysaccharide generation kinetic model and substrate consumption kinetic model which describe fermentation process of white-rot fungi were established. Meanwhile, the experimental data were verified by this model, and a good fitting result with an average relative error less than 10% between the data obtained from experiments and the model was yielded. The results show that these models can predict the growth and metabolic rules of white-rot fungus, the fermentation process of exopolysaccharides and the kinetic mechanism of white-rot fungus accurately.To investigate the growth conditions of white-rot fungus and simulate its metabolism kinetic models, the rules how the factors such as biomass, culture fluid, pH value, glucose consumption and exopolysaccharides generation, etc., changed during the batch culture process of white-rot fungi by using an air-lift fermenter, as well as metabolic kinetics of white-rot fungi were studied. Based on Logistic equation, Luedeking-Piret equation and experimental data, the correlation model parameters of mycelia biomass, glucose consumption and exopolysaccharide generation were obtained and found to be change with time in metabolism process. Detailedly, μm=0.071 8 h-1,α= 0.831 8 g/(g·h), β= 0.002 g/(g·h), b1=0.016 3 g/(g·h) and b2=3.023 3 g/(g·h). Hence the mycelial growth kinetic model, exopolysaccharide generation kinetic model and substrate consumption kinetic model which describe fermentation process of white-rot fungi were established. Meanwhile, the experimental data were verified by this model, and a good fitting result with an average relative error less than 10% between the data obtained from experiments and the model was yielded. The results show that these models can predict the growth and metabolic rules of white-rot fungus, the fermentation process of exopolysaccharides and the kinetic mechanism of white-rot fungus accurately.

关 键 词：WHITE-ROT fungus(Phanerochaete chrysosporium) Exopolysaccharides (EPS) METABOLIC kinetic MODEL MODEL parameter Logistic EQUATION Luedeking-Piret EQUATION 

分 类 号：S476[农业科学—农业昆虫与害虫防治]