Effects of Artificially Designed shRNA of chTR on Telomerase Activity  

端粒酶RNA亚基的shRNA对细胞端粒酶活性的影响(英文)

作　　者：张哲[1,2] 明宇[1,2] 于聪[3] 张玉静[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学人兽共患病研究所,吉林长春130062 [3]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《Agricultural Science & Technology》2008年第6期51-54,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.30471284)~~

摘　　要：[Objective] The study was to investigate the inhibitory effects of shRNA of chTR on telomerase activity in MDCC-MSB1 cells. [Method] The artificially designed and synthesized short hairpin RNA(shRNA) of chicken telomerase RNA(chTR) was used to construct the expression vector that was subsequently transfected to MDCC-MSB1 cells, then the telomerase activity of these cells measured by improved TRAP method. [Result] Compared to the CK group, the telomerase activities in the treatment cells showed no obvious decrease in 24 h after transfection, while decreased remarkably 48 h later, among which in the cells transfected by interference vector pSi-chTR-sh1 presented the most obvious inhibitory effects. [Conclusion] This vector could effectively inhibit the telomerase activities in MDCC-MSB1 cells.[Objective] The study was to investigate the inhibitory effects of shRNA of chTR on telomerase activity in MDCC-MSB1 cells. [Method] The artificially designed and synthesized short hairpin RNA(shRNA) of chicken telomerase RNA(chTR) was used to construct the expression vector that was subsequently transfected to MDCC-MSB1 cells, then the telomerase activity of these cells measured by improved TRAP method. [Result] Compared to the CK group, the telomerase activities in the treatment cells showed no obvious decrease in 24 h after transfection, while decreased remarkably 48 h later, among which in the cells transfected by interference vector pSi-chTR-sh1 presented the most obvious inhibitory effects. [Conclusion] This vector could effectively inhibit the telomerase activities in MDCC-MSB1 cells.

关 键 词：TELOMERASE TELOMERASE RNA SHRNA 

分 类 号：S188[农业科学—农业基础科学]