Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences  

作　　者：LI ZiHui1,2, FENG XianMin1, LU SiQi1, ZHANG Fan1, WANG FengYun1 & HUANG Song1 1 Department of Pathogenic Biology, Capital Medical University, Beijing 100069, China 2 Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101100, China 

出　　处：《Science China(Life Sciences)》2008年第5期445-452,共8页中国科学（生命科学英文版）

基　　金：Supported by the Natural Science Foundation of Beijing, China (Grant No. 7052009);the National Natural Science Foundation of China (Grant No. 30470243)

摘　　要：To clarify the phylogenetic relationships and species status of Pneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) of Pneumocystis rRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of six Pneumocystis species compared with other fungi like Taphrina and Saccharomyces indicated that the Pneumocystis genus contained multiple species including Pneumocystis from gerbil. The phylogenetic tree also showed that Pneumocystis from human and monkey formed one group and four rodent Pneumocystis formed another group. Among the four members, Pneumocystis wakefieldiae was most closely related to Pneumocystis murina and Pneumocystis carinii, and was least related to gerbil Pneumocystis.To clarify the phylogenetic relationships and species status of Pneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) of Pneumocystis rRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of six Pneumocystis species compared with other fungi like Taphrina and Saccharomyces indicated that the Pneumocystis genus contained multiple species including Pneumocystis from gerbil. The phylogenetic tree also showed that Pneumocystis from human and monkey formed one group and four rodent Pneumocystis formed another group. Among the four members, Pneumocystis wakefieldiae was most closely related to Pneumocystis murina and Pneumocystis carinii, and was least related to gerbil Pneumocystis.

关 键 词：PNEUMOCYSTIS PHYLOGENY 5.8S RRNA GENE internal transcribed SPACERS 

分 类 号：Q75[生物学—分子生物学]