Isolation of Chlorella vulgaris and Its DNA Extraction Methods  

小球藻的分离及其DNA提取方法的研究(英文)

作　　者：王恒强[1] 孔庆军[1] 任雪艳[1] 占东霞[1] 张海黎[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《Agricultural Science & Technology》2008年第4期44-46,共3页农业科学与技术（英文版）

基　　金：Supported by the High-level Talents Start-up Fund of Shihezi University(07002-500002061401)~~

摘　　要：[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.

关 键 词：CHLORELLA VULGARIS ISOLATION METHOD DNA EXTRACTION METHOD 

分 类 号：Q943[生物学—植物学]