中风易感性与候选基因SNP位点关联研究  

Association Study on Relationship of Stroke-vulnerability and SNPs of Candidate Genes

作　　者：张小燕 胡木林[2] 周才秀[3] 王忠[2,3] 陈沁 

机构地区：[1]上海芯超生物科技有限公司,上海201203 [2]中国中医科学院西苑医院,北京100091 [3]中国中医科学院中医临床基础医学研究所,北京100700 [4]上海博星基因芯片有限责任公司,上海200433

出　　处：《生物技术通报》2008年第S1期165-171,175,共8页Biotechnology Bulletin

基　　金：国家自然基金课题(中医药重大研究计划)(90209015);国家自然基金课题(30672577)

摘　　要：脑中风是严重危害我国中老年人身体健康的主要疾病之一,对中风相关基因单核苷酸多态性位点在人群中分布情况的研究将有助于深入认识中风发病机制及提高防治能力。该研究用寡核苷酸芯片方法检测了8个多态性位点与中风易感性之间的关联。结果发现:寡核苷酸芯片技术作为一种很好的基因分型方法,具有高通量、平行性、微量化、自动化和快速灵敏等的特点,适用于大规模基因分型平台的建立;实验初步发现与中风易感性相关的有血管紧张素原(AGT)基因M235T(OR=3.437,CI=1.211,9.709),载脂蛋白E(APOE)基因cys158arg (OR=9.434,1.686-83.3),亚甲基四氢叶酸还原酶(MTHFR)基因C677T(OR=2.591,1.002-6.711),血管紧张素ⅡⅠ型受体(AT1R)基因A1166C(OR=0.213,0.057-0.799)。Stroke is one of the most threatening diseases to the health of middle-aged and aged people.The research on distributions of the SNP loci of stroke-related genes in populations is helpful to elucidate the mechanism of stroke and improve the therapy of it.In the present study,eight SNP loci were analyzed by oligonucleotide microarray and the association between the SNP loci and the vulnerability of stroke were studied.The result showed that,as an excellent genotyping method, oligonucleotide microarray owns the high-throughout,parallel,fast and sensitive character and is suitable to the construction of large-scale table of genotyping.In the eight tested polymorphism loci,we found four SNPs including angiotensinogen (AGT)M235T site,apolipoprotein E(APOE)cys158arg site,methyllenetetrahydrofolate reductase(MTHFR) C677T site,angiotensinⅡtypel receptor(AT1R)A1166C site were statistically associated with the increased risk of stroke.Further exploration and research of the association between SNPs and stroke were expected on a larger scale.

关 键 词：寡核苷酸芯片技术 单核苷酸多态性 中风易感性 

分 类 号：R743[医药卫生—神经病学与精神病学]