密码子优化小鼠socs-1基因及其在大肠杆菌中的表达  

Codon Optimization of Mouse socs-1 and its Expression in E.coli

作　　者：龚桂花[1] 刘艳[1] 胡又佳[1] 朱春宝[1] 朱宝泉[1] 

机构地区：[1]上海医药工业研究院创新药物与制药工艺国家重点实验室(筹),上海200437

出　　处：《生物技术通报》2008年第S1期291-295,共5页Biotechnology Bulletin

摘　　要：根据已报道的小鼠socs-1基因序列,依据大肠杆菌密码子偏爱性,设计并采用重叠PCR法合成了全长693bp的socs-1基因,将其克隆到表达载体pET-22b中构建表达载体pET-SOCS1。经IPTG诱导后,SDS-PAGE电泳显示此密码子优化后的基因可在大肠杆菌BL21(DE3)中高效表达。According to the codon bias of E.coli,the gene encoding Suppressor of Cytokine Signaling-1(SOCS-1) was optimized and artificially synthesized by PCR without changing the amino acid sequence.The recombinant plasmid pET-SOCS1,which contains the modified socs-1 sequence,was then constructed and transformed in E.coli BL21(DE3). After IPTG induction,recombinant SOCS-1 could be successfully expressed at a high level in E.coli.

关 键 词：SOCS-1 密码子优化 重叠PCR 

分 类 号：Q78[生物学—分子生物学]