腺苷产生菌Xn151菌株的选育及发酵条件研究  

Breeding of and Fermentation an Adenosine-producing Strain Xn151

作　　者：施庆珊[1] 林小平[1] 许虹[1] 疏秀林[1] 冯静[1] 陈爱美[1] 邱玉棠[1] 欧阳友生[1] 陈仪本[1] 

机构地区：[1]广东省微生物研究所广东省微生物应用新技术公共实验室,广州510070

出　　处：《生物技术通报》2008年第S1期425-430,共6页Biotechnology Bulletin

基　　金：广州市科技计划项目(2006Z3-E0601);广东省科技攻关项目(2002C1040103)资助

摘　　要：以肌苷产生菌枯草杆菌GMI-741(Ade^(--))为出发菌株,通过多因子诱变选育出具有黄嘌呤、鸟嘌呤双重营养缺陷型、丧失腺嘌呤脱氨酶的腺苷产生菌Xn151。以Xn151为发酵菌株,采用正交实验设计法对其发酵基础培养基进行优化,确定最佳发酵配方。利用此培养及配方摇瓶发酵72h,腺苷产量可达12.3g/L。The induction of adenosine-producing strain from an inosine-producing strain Bacillus substilis GMI-741 was attempted,and it was found out that a diauxotrophi mutant(xanthine and guanine-requiring)Bacillus substilis Xn151 lacking of adenase produce a large amount of adenosine.The orthogonal factorial design was carried out,to optimize the culture medium.The optimal medium,based on statistical analysis,showed a production of 12.3 g/L adenosine on a reciprocal shaker for 72 h.

关 键 词：腺苷 菌种选育 发酵 正交实验设计 

分 类 号：Q93[生物学—微生物学]