底泥中芘高效降解菌株的富集分离及特性研究  

Screening and Characterization of Highly Efficient Pyrene-degrading Bacteria from Sediment

作　　者：姜世英[1,2] 张颖[1] 林俊杰[3] 王新新[1] 王元芬[1] 

机构地区：[1]中国科学院沈阳应用生态研究所中国科学院陆地生态过程重点实验室,沈阳110016 [2]东北大学环境科学专业,环境科学专业沈阳110004 [3]吉林农业大学资源与环境学院,长春130000

出　　处：《生物技术通报》2008年第S1期441-444,450,共5页Biotechnology Bulletin

基　　金：国家重点基础研究发展规划资助项目(2004CB418505);国家自然科学基金项目(30670391)

摘　　要：从辽河流域浑河沈阳段采集底泥样品,利用吸附性载体,以芘为唯一碳源,筛选到一株芘高效降解菌F8,根据形态。生理生化特性及测序结果鉴定该菌为产碱杆菌(Alcaligenes sp.)。对菌株F8降解率进行测定,结果表明,菌株F8在32℃振荡培养条件下,7d后对50 mg·L^(-1)的芘降解率为62.75%,芘的降解与细菌浓度的增长呈正相关关系?通过对其培养条件优化,确定其生长的最适温度和pH分别为36℃和6.5。对菌株F8在重金属离子胁迫下的生长研究发现,Cd^(2+)对菌株F8有毒性;Zn^(2+)对菌株有一定的抑制作用;Cu^(2+)对菌株生长影响很小。研究了植物-微生物联合修复作用,结果显示水稻促进了菌株F8对芘的降解,使降解率增加了11.85%。A highly efficient pyrene-degrading bacteria was isolated from the sediment in Hunhe River of Liaohe Basin in Shenyang city,and one strain F8 identified as Alcaligenes sp.was chosen for use.The study on the degradability showed that at 32℃,the degradation rate of pyrene(50 mg·L^(-1))was 62.75%after 7 days shake culture.A significant positive relationship Was found between bacterial growth and pyrene degradation.The strain was suggested that the optimization of culture condition was:36℃,pH6.5.Cd^(2+)was toxic to the strain,Zn^(2+)had inhibiting effect;while Cu^(2+)had no significant effect.Phyto-micro Combined Remediation on Polluted Sediment was studied,results show that the rice promoted the degrading of pyrene,and the degradation rate increased by 11.85%.

关 键 词：生物降解 芘 产碱杆菌 培养条件 植物-微生物联合修复 

分 类 号：X172[环境科学与工程—环境科学]