纳升电喷雾串联质谱在阿托西班一级结构确证中的应用  

Confirmation of the Primary Structure of Atosiban by Nanoelectrospray Tandem Mass Spectrometry

作　　者：李萍[1] 王红霞[1] 王鸿丽[1] 刘炳玉[1] 刘锋[1] 薛燕[1] 魏开华[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《分析测试学报》2008年第S1期16-18,共3页Journal of Instrumental Analysis

摘　　要：The primary structure of atosiban(an artificially synthesized 8AA peptide) was confirmed by nanoelectrospray tandem mass spectrometry.Accurate relative molecular mass of atosiban unreduced and reduced were determined firstly by full-scan mass spectrometry,and fragmentation ions were obtained by selecting parental ions m/z 498.73(double charge) using mass spectrometry(MS/MS).The sequence and modified site of atosiban was designated by using y ion method.The method is sensitive,fast and simple,and can provide a great valuable information for confirmation of primary structure of polypeptide drugs.The primary structure of atosiban(an artificially synthesized 8AA peptide) was confirmed by nanoelectrospray tandem mass spectrometry.Accurate relative molecular mass of atosiban unreduced and reduced were determined firstly by full-scan mass spectrometry,and fragmentation ions were obtained by selecting parental ions m/z 498.73(double charge) using mass spectrometry(MS/MS).The sequence and modified site of atosiban was designated by using y ion method.The method is sensitive,fast and simple,and can provide a great valuable information for confirmation of primary structure of polypeptide drugs.

关 键 词：Nano-ESI MS/MS ATOSIBAN DISULFIDE BOND modified SITE 

分 类 号：R914[医药卫生—药物化学]