大鼠尿液中硝基安定代谢物的GC-MS检验  

Determination of the Metabolites of Nitrazepam in Rat Urine with GC-MS

作　　者：黄克建[1] 潘智文[2] 李宏森[1] 林翠梧[2] 刘晓锋[1] 罗正坚[1] 李璐[1] 陈而廉[3] 朱定姬[2] 

机构地区：[1]广西壮族自治区公安厅物证鉴定中心,广西南宁530012 [2]广西大学化学化工学院,广西南宁530004 [3]广西壮族自治区公安厅科技处,广西南宁530004

出　　处：《分析测试学报》2008年第S1期28-30,共3页Journal of Instrumental Analysis

基　　金：公安部应用创新计划资助项目(2007YYCXGXST081)

摘　　要：The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.

关 键 词：NITRAZEPAM URINE METABOLITE solid phase extraction gas chromatography-mass SPECTROMETRY 

分 类 号：R114[医药卫生—卫生毒理学]