人血清中脂肪酸的GC-MS分析  

Analysis of Fatty Acids Compositions in Human Serum by GC-MS

作　　者：李海静[1] 吴胜明[1] 李昱[1] 方均建[1] 马昆鹏[1] 宋占军[1] 董方霆[1] 

机构地区：[1]国家生物医学分析中心,北京100850

出　　处：《分析测试学报》2008年第S1期37-38,共2页Journal of Instrumental Analysis

摘　　要：A method for determination of fatty acids(FA) compositions in human serum by gas chromatography time-of flight mass spectrometry(GC-TOF MS) method was established.The FA in serum were directly transformed into the fatty acid methyl esters by 2.5% H2SO4-methanol.Heptadecanoic acid methyl esters were used as internal standard.The detection and separation of all the FA were performed within 33 min.The established method has the characteristic of simple operation,rapid analysis,high accuracy,large amount of information and safety.So it is suitable for the scientific research to the disease correlating FA.A method for determination of fatty acids(FA) compositions in human serum by gas chromatography time-of flight mass spectrometry(GC-TOF MS) method was established.The FA in serum were directly transformed into the fatty acid methyl esters by 2.5% H2SO4-methanol.Heptadecanoic acid methyl esters were used as internal standard.The detection and separation of all the FA were performed within 33 min.The established method has the characteristic of simple operation,rapid analysis,high accuracy,large amount of information and safety.So it is suitable for the scientific research to the disease correlating FA.

关 键 词：FATTY ACIDS human serum gas CHROMATOGRAPHY time-of flight mass spectrometry(GC-TOF MS) H2SO4-methanol 

分 类 号：R446.1[医药卫生—诊断学]