ECD & CAD裂解方式的FT-ICR-MS研究  

Study on ECD & CAD Dissociation Using FT-ICR-MS Method

作　　者：石磊[1] 程舸[1] 于雷[1] 刘晓梅[1] 王韶[1] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021

出　　处：《分析测试学报》2008年第S1期53-54,共2页Journal of Instrumental Analysis

基　　金：吉林省科技厅白求恩医学专项(200705391)

摘　　要：FT-ICR-MS technology has significant sensitivity and dynamic range,enabling the detection of different fragmentations of peptides and proteins at lower and lower levels.In the bottom-up approach,by far the most frequently used proteomics approach,a single LC-MS run can reveal tens and even hundreds of useful information.For each peptide selected for MS/MS,two data-files were generated,one for ECD and another one for CAD.They were merged as described and submitted to the Mascot search engine using the protein database.These thresholds ensure almost 100% statistical confident in the peptide identification.FT-ICR-MS technology has significant sensitivity and dynamic range,enabling the detection of different fragmentations of peptides and proteins at lower and lower levels.In the bottom-up approach,by far the most frequently used proteomics approach,a single LC-MS run can reveal tens and even hundreds of useful information.For each peptide selected for MS/MS,two data-files were generated,one for ECD and another one for CAD.They were merged as described and submitted to the Mascot search engine using the protein database.These thresholds ensure almost 100% statistical confident in the peptide identification.

关 键 词：ECD CAD FOURIER TRANSFORM ion CYCLOTRON resonance mass spectrometry(FT-ICR-MS) 

分 类 号：TH843[机械工程—仪器科学与技术]