解盐促生菌Rs-5溶磷动力学及其机制  

Kinetics and mechanism of phosphorus dissolved by strain Rs-5 for relief-salt stress and growth promotion

作　　者：石在强[1] 岳海涛[1] 郑元元[1] 李晖[1] 姚丽霞[1] 李春[1,2] 

机构地区：[1]石河子大学农学院新疆兵团化工绿色过程重点实验室,石河子832003 [2]北京理工大学生命科学与技术学院,北京100081

出　　处：《农业工程学报》2008年第S1期24-28,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(No.20776017);新疆维吾尔自治区高新技术重点项目(200811108);石河子大学自然科学研究与技术创新项目(No.ZRKX2005024)

摘　　要：以次生盐渍化土壤中难溶性无机磷Ca3(PO4)2为磷源,利用钼锑抗比色法测定溶磷特性,高效液相色谱(HPLC)测定有机酸,研究了解盐促生菌Rs-5溶磷动力学及其溶磷机制。结果表明:菌株Rs-5溶磷量随菌体生长而逐渐增加,第4d溶磷量达到最大79.86mg/L,当Ca3(PO4)2在一定浓度范围时,溶磷量与菌体量呈显著正相关。溶磷过程中菌株Rs-5培养液pH值随菌体生长而逐渐降低,说明菌株Rs-5溶磷过程产生酸类物质,有机酸检测结果表明主要为柠檬酸和苹果酸,且其含量与溶磷量呈显著正相关。说明菌株Rs-5的溶磷动力学模型为生长耦联型,且产柠檬酸及苹果酸是其溶磷的主要原因。Phosphorus,dissolved by relieving salt and growth promoting strain Rs-5,was determined with the method of molybdenum blue colorimety using Ca3(PO4)2 as P resource,the major type of inorganic P that did not dissolve easily in alkali-saline soil.The organic acids were determined with high performance liquid chromatogram(HPLC).The results indicated that P dissolving was associated with the growth of bacterium Rs-5.The maximum dissolved P value reached 79.86mg/L on the fourth day.The dissolved P value showed positive relationship with the biomass when the concentration of Ca3(PO4)2 was confirmed.The pH value in medium was decreased with the growth of bacterium Rs-5. It was indicated that some acid substances were produced.The results of organic acids measured were shown that citric acid and malic acid,produced mainly by strain Rs-5 were the primary reasons of P dissolving,which had direct correlation to the dissolved P.It was testified that the P dissolving model of bacterium Rs-5 was growth-associated and the citric acid and malic acid,produced mainly by strain Rs-5 were primary reasons.

关 键 词：解盐促生 溶磷 有机酸 盐胁迫 细菌 

分 类 号：S182[农业科学—农业基础科学]